摘要
目的:验证阳陵泉穴对艾灸治疗类风湿关节炎的抗炎增效作用,探讨少阳对骨之作用机制。方法:佐剂性关节炎大鼠40只,按治疗用穴不同,随机分为空白组;模型组;肾俞、足三里组;阳陵泉、肾俞、足三里组;太溪、肾俞、足三里组。各组造模后第7天,每日行小艾条温和灸,每组各穴单侧灸治5 min,每日1次,左右交替进行,6 d为1个疗程,共3个疗程。(1)ELISA法检测各组血清TNF-α、IL-6、NO水平;(2)RT-PCR法检测各组滑膜组织TNF-α mRNA表达。结果:艾灸阳陵泉、肾俞、足三里组血清TNF-α、IL-6、NO水平及滑膜组织TNF-α mRNA表达分别较肾俞、足三里组和太溪、肾俞、足三里组降低,差异有统计学意义(P<0.05)。结论:足少阳胆经阳陵泉穴对艾灸肾俞、足三里穴治疗佐剂性关节炎大鼠具有明确抗炎增效作用,提示少阳与骨存在密切生理联系。
Objective ： To explore the anti - inflammation of Yanglingquan （ GB34 ） with the moxibustion treatment and the mechanism of Shaoyang meridian in treating bones and joints disease. Methods ： Forty adjuvant arthritis rats modulated by complete freund＇s adjuvant（CFA） were randomly divided into control group, model group, Shenshn （BL23） and Zusanli（ST36） group, Yanglingquan （ GB34 ） , Shenshu （ BL23 ） and Zusanli （ ST36 ） group, Taixi （ KI3 ）, Shenshu （ BL23 ） and Zusanli （ ST36 ） group. The treatment began seven days after modeling and moxibustion was applied on each acupoint by moxa cone 5 rain for each acuponts, once a day and the acupoints were chosen alternately,6 days for 1 course,continued 3 courses. To detect TNF - α, IL -6 and NO contents in serum of rats by ELISA method. And the expression of TNF - cx mRNA was tested by real time fluorescence quantita- tive PCR in synovial tissue in rats. Results：The inhibition effects on the TNF - α,IL -6 and NO in serum and TNF - α mRNA in the synovial tissues showed that Yangiingquan （ GB34 ） , Shenshu （ BL23 ） Zusanli （ ST36 ） group was the most significant effect group compared with Shenshu（ BL23 ） and Zusanli（ST36） group and Taixi （ KI3 ）, Shenshu （ BI23 ） and Zusanli （ ST36 ） group. The contents of TNF - ct,IL - 6,NO in serum and TNF - α mRNA in the synovial tissues decreased in different degrees （ P 〈 0. 05 ）. Conclusion ： Moxihustion on Yanglingquan（GB34） shows a synergistic effect on anti - inflammation. It reveals the close re- lationship between Shaoyang and bones.
出处
《辽宁中医杂志》
CAS
北大核心
2017年第4期852-854,共3页
Liaoning Journal of Traditional Chinese Medicine
基金
国家自然科学基金项目(81373738)
四川省教育厅科研基金项目(14ZB0100)
四川省教育厅中国盐文化研究中心项目(YWHY-13-05)
关键词
少阳
骨
阳陵泉
佐剂性关节炎
抗炎增效
Shaoyang
bones
Yanglingquan （ GB34 ）
adjuvant arthritis
synergistic effect of anti - inflammatory treatment
