摘要
目的观察复心合剂对心力衰竭大鼠β_1-肾上腺素能受体抗体(β_1-adrenergic receptor,β_1-AR)-环腺苷酸环化酶(cyclic adenosine monophosphate,c AMP)-蛋白激酶A(protein kinase A,PKA)通路的影响。方法将成年雄性Wistar大鼠随机分为空白对照组、卡托普利组、复心合剂(中药)低剂量组、高剂量组及模型组。建立心力衰竭大鼠模型,干预6周后,分离大鼠左室,分析左室质量指数(left ventricular mass index,LVMI),检测心肌组织β_1-AR mRNA表达,测定大鼠血浆c AMP水平和大鼠脾组织匀浆OD值及PKA含量。结果与空白对照组比较,模型组大鼠LVMI、血浆c AMP升高(P<0.05),β_1-AR mRNA表达、脾组织匀浆OD值及PKA降低(P<0.01)。与模型组比较,中药高剂量组、卡托普利组LVMI降低(P<0.01),β_1-AR mRNA相对表达量升高(P<0.05);各给药组大鼠血浆c AMP水平降低(P<0.01),脾组织匀浆OD值、PKA表达升高(P<0.01)。与卡托普利组比较,中药低剂量组β_1-AR mRNA相对表达量、脾组织匀浆OD值及PKA表达降低(P<0.01),LVMI及c AMP水平升高(P<0.05)。与中药低剂量组比较,中药高剂量组LVMI、cAMP水平降低(P<0.05),β_1-AR mRNA相对表达、脾组织匀浆OD值及PKA表达升高(P<0.01)。结论复心合剂通过调节β_1-AR-cAMP-PKA通路发挥抗心力衰竭作用。
Objective To observe the effect of Fuxin Mixture（FXM） on the β1-AR（adrenergic re- ceptor）-cAMP（cyclic adenosine monophosphate, cAMP）-PKA （ protein kinase A, PKA ） pathway of rats with heart failure. Methods Male Wistar rats were randomly divided into blank control group, captopril group, FXM low dose group,FXM high dose group and model group.Models of CHF were established. After drug intervention for 6 weeks, the left ventricular mass index（LVMI） was analysed, the expression of β1- AR mRNA in myocardial tissue was measured ,the level of cAMP in rat plasma ,the OD value PKA content of spleen tissue homogenate were detected. Results Compared with the blank control group, the LVMI and the cAMP in plasma of model group were increased （P 〈0.05）, the expression of β1-AR mRNA, the OD value of spleen tissue homogenate and PKA were decreased （P 〈0.01 ）. Compared with the model group, the LVMI were decreased,and the expression ofβ1-AR mRNA were increased in FXM high dose group and captopril group （P 〈0.01, P 〈0.05） ; the level of cAMP in plasma of each drug group were de- creased （P〈0. 01 ）, the OD value of spleen tissue homogenate and PKA were increased （P 〈0.01 ）. Compared with the captopril group, the expression of β1-AR mRNA, the OD value of spleen tissue ho- mogenate and PKA were decreased, and the LVMI and the cAMP were increased in the FXM low dosegroup （P 〈0.01, P 〈0.05）. Compared with the FXM low dose group, the LVMI and the cAMP of FXM high dose group were decreased （P 〈0.05）, the expression of β1-AR mRNA, the OD value of spleen tissue homogenate and PKA were increased （P 〈0.01 ）. Conclusion FXM could play the role of anti-heart fail- ure through regulating β1-AR-cAMP-PKA pathway.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2017年第4期453-457,共5页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家自然科学基金资助项目(No.81273703)
