摘要
从福建平潭海域、台湾海峡等地采集海泥、海水样品,从中筛选得到1株产α-淀粉酶的菌株825,经16S rDNA鉴定为芽孢杆菌属。优化其产酶培养基,得到发酵培养基配方:可溶性淀粉2.0%,酵母膏0.5%,蛋白胨1.5%,培养基初始pH 7.0,发酵周期60 h。经优化后,α-淀粉酶活力从46.55 U/m L提高到77.44 U/m L,提高了66.36%。对其酶学性质的研究表明,该酶的最适反应温度70℃,在30~50℃稳定性较好,保温1 h仍可保持90%以上的酶活力;最适反应pH 7.0,在pH 5.0~9.0的缓冲液中保温1 h,相对酶活保持在70%以上,说明具有较宽的pH作用范围。
Strain 825 with α-amylase activity was isolated from marine samples of Pingtan sea and Tai wan strait and identified as Bacillus sp.by 16S rDNA sequence analysis.Amylase-producing medium of strain 825 was optimized,and the optimum conditions were as follow：soluble starch 2.0%,yeast extract 0.5%,peptone 1.5%,initial pH 7.0,fermentation period 60 h.Under these conditions,α-amylase activity increased from 46.55 U/mL to 77.44 U/mL,66.36% higher than before.Furthermore,the α-amylase properties were analyzed and the results were obtained.The optimal reaction temperature of α-amylase was 70 ℃,and more than 90% of its maximum activity was maintained after incubation for 1h under 30-50 ℃.The optimal reaction pH was 7.0,and more than 70% activity was retained after incubation for 1h in the pH 5.0-9.0.It indicated that this α-amylase had a wide pH tolerance range.
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2017年第2期77-84,共8页
Journal of Chinese Institute Of Food Science and Technology
基金
国家863计划课题(2013AA102101)
国家海洋公益性行业科研专项(201305015)
关键词
Α-淀粉酶
海洋微生物
筛选鉴定
产酶条件优化
酶学性质
α-amylase
marine microorganism
isolation and identification
optimization of fermentation conditions
enzymatic properties
