摘要
目的:建立多波长同时测定地锦草药材中没食子酸、鞣花酸、槲皮素、山柰酚4种指标成分含量的超高效液相色谱法,并比较不同地锦草化学成分的差异。方法:采用Acquity UPLC BEH C_(18)色谱柱(50 mm×2.1 mm,1.7μm),以乙腈-0.2%磷酸水溶液为流动相,梯度洗脱,柱温30℃,流速0.2 mL·min^(-1),检测波长为271 nm(0~2 min)、254 nm(2~6 min)和360 nm(6~8 min)。结果:地锦草中没食子酸、鞣花酸、槲皮素、山柰酚4种成分在8 min内均达到基线分离,线性范围分别为65.88~164.70(R^2=0.999 2)、149.10~372.75(R^2=0.999 2)、27.48~68.70(R^2=0.999 7)、30.24~75.60μg·mL^(-1)(R^2=0.999 2),平均回收率(n=6)分别为99.5%(RSD=1.4%)、98.3%(RSD=0.92%)、99.1%(RSD=1.2%)和99.3%(RSD=1.4%);6批地锦草样品中没食子酸、鞣花酸、槲皮素、山柰酚的含量分别为4.148~7.663、9.727~17.388、1.763~2.977、0.684~2.037 mg·g^(-1)。结论:本文对不同产地地锦草中指标性成分含量差异性的研究,可为地锦草的质量控制提供理论依据。
Objective： To establish a multi-wavelength UPLC method for simultaneous determination of four index components （ gallic acid, spent acid, quercetin and kaempferol ） in Euphorbiae Humifusae Herba, and to compare the difference among samples from different habitants. Methods： The analysis was performed on a 30 ℃ thermostatic Acquity UPLC BEH Cls column （ 50 mm× 2.1 mm, 1.7 μm ）, with the mobile phase consisting of acetonitrile and 0.2% phosphoric acid by gradient elution. The flow rate was 0.2 mL · min^-1 and the detection wavelengths were 271 nm（ 0-2 min ）, 254 nm（ 2-6 min ） and 360 nm（ 6-8 rain ）. Results： Baseling resolved peaks were achieved for four components in 8 minutes. The linear ranges of gallic acid, spent acid, quercetin and kaempferol fell within the ranges of 65.88-164.70 μg·mL^-1（R2=0.999 2 ）, 149.10-372.75 μg·mL^-1 （ R2=0.999 2 ）, 27.48-68.70 μg·mL^-1 （ R2-0.999 7 ） and 30.24-75.60 μg·mL^-1（ R2=0.999 2 ）, respectively. The average recoveries （ n=6 ） were 99.5% （ RSD=1.4% ）, 98.3% （ RSD=0.92% ）, 99.1% （ RSD=1.2% ） and 99.3% （ RSD=1.4% ）, respectively. The contents of four components in 6 batches for samples were 4.148-7.663 mg·mL^-1, 9.727-17.388 mg·mL^-1, 1.763-2.977 mg·mL^-1and 0.684-2.037 mg· g^-1, respectively. Conclusion： This paper revealed difference in contents of indicative components in Euphorbiae Humifusae Herba, thus providing a theoretical basis for the quality control of the herb.
作者
彭纪铭
路娟
陈哲
王同瑾
李伟
陈曦
PENG Ji-ming LU Juan CHEN Zhe WANG Tong-jin LI Wei CHEN Xi(Shandong University of Traditional Chinese Medicine, Jinan 250355, China Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China)
出处
《药物分析杂志》
CAS
CSCD
北大核心
2017年第4期589-594,共6页
Chinese Journal of Pharmaceutical Analysis
基金
国家自然科学基金面上项目(81673667)
国家重大科技专项(2014ZX09201022-006)
