摘要
目的:建立测定抗风湿镇痛类健康产品中非法添加吲哚美辛含量的高效液相色谱(HPLC)方法。方法:选用InertSustain C_(18)(4.6mm×250mm,5μm)色谱柱,以乙腈-0.02mol·L^(-1)乙酸铵溶液为流动相进行梯度洗脱,流速为1.0mL·min^(-1),检测波长为266nm,柱温为35℃。结果:吲哚美辛在1.010~202.1μg·mL^(-1)浓度范围内与峰面积呈良好的线性关系,相关系数r=0.999 7,加样回收率为98.1%。13批供试品检测到非法添加吲哚美辛。结论:该方法专属性强、灵敏度高,适用于抗风湿镇痛类健康产品中非法添加吲哚美辛的含量测定。
Objective:To establish a HPLC method for the detection of indometacin illegally added into antirheumatic pain relieving healthy products.Methods:InertSustain C18(4.6mm×250mm,5μm)was adopted with the mobile phase of acetonitrile and 0.02mol·L^(-1) ammonium acetate by gradient elution.The flow rate was 1.0mL·min^(-1),the detection wavelength was 266 nm and the column temperature was 35°C.Results:Indometacin showed a good linearity in the range of 1.010~202.1μg·mL^(-1),r=0.9997,and its average recovery rate was 98.1%.Indometacin was found in thirteen samples.Conclusion:The method is selective and sensitive and can be employed in the detection of indometacin in antirheumatic pain relieving health products.
出处
《亚太传统医药》
2017年第8期44-46,共3页
Asia-Pacific Traditional Medicine
