摘要
Hp的液体培养尤其是大规模培养仍存在许多问题。通过单因子试验统计分析,优化筛选了适于幽门螺杆菌的液体培养基和摇瓶培养条件。结果表明,在适宜的摇瓶培养条件下,Hp能够在添加β-环糊精(β-Cyclodextrin,β-CD)的布氏肉汤中很好地生长,菌体收获量达到0.6g/mL。
There are still many problems at liquid state culture especially large-scale culture for Helicobacter pylori at present. Using analytical technique of single factor test statistics, a suitable medium and shaking culture conditions for Helicobacter pylori have been selected. The experimental results showed: under the optimal shaking culture conditions Hp could be growed well enough in Brucella broth with β-CD. The thalli weight reached 0.6g/100mL.
出处
《微生物学通报》
CAS
CSCD
北大核心
2002年第4期34-37,共4页
Microbiology China
基金
国家"九五"重点科技攻关计划课题(No.96.96-901-01-54)
关键词
单因子试验
幽门螺杆菌
液体培养工艺
Helicobacter pylori, Liquid state culture, Single factor test
