摘要
怀牛膝多糖是常用中药牛膝中的主要活性成分,可作为一种免疫调节剂,是重要的多糖药物之一。本实验采用微波辅助法提取怀牛膝中多糖,溶剂为水,采用苯酚-硫酸比色法测定怀牛膝多糖含量,通过单因素及正交试验研究微波功率、料液比、提取温度和微波作用时间对怀牛膝多糖提取率的影响。结果表明,怀牛膝多糖微波提取的最佳工艺条件为:微波功率为600W、料液比1:20(g/m L)、浸取温度为70℃、微波处理时间4min。
Achyranthes bidentata polysaccharide is one of the main active ingredient in traditional Chinese medicine Achyranthes bidentata, and it is one of the most important polysaccharide drugs, which can be used as an immunomodulator. Microwave extraction was used to extract polysaccharide from Achyranthes bidentata in this article, using water as solvent. The influence factors on extraction yield were studied by single factor and orthogonal experiment, which included microwave power, the solid-liquid ratio, extraction temperature and microwave-heating time. The results showed that the optimal conditions were as follows： microwave power 600W, the 1：20 （g/mL） ratio of solid to liquid, extracting at 70℃ for 4min.
出处
《中国果菜》
2017年第4期16-19,共4页
China Fruit & Vegetable
基金
2015年河南省高等学校重点科研项目(15A180065)
关键词
怀牛膝
多糖
微波提取
Achyranthes jbidentata
polysaccharide
microwave extraction
