摘要
目的:探讨基质金属蛋白酶MMP-9、MMP-13及其抑制剂TIMP-1、TIMP-3在脊柱结核椎间盘组织中的表达及意义。方法:选择2012年1月至2016年1月期间,在我院行手术治疗的48例脊柱结核患者作为TB组,48例行手术治疗的椎间盘突出患者作为ID组,并选择同期在我院接受治疗的外伤性脊柱骨折患者作为对照组,采用免疫组化检测法及实时荧光定量PCR(RT-PCR)技术,比较三组患者的阳性率、表达水平。结果:TB组的MMP-9的阳性率为93.75%、MMP-13的阳性率为89.58%、TIMP-1的阳性率为87.50%、TIMP-3阳性率为91.67%均明显高于ID组(70.83%、66.67%、68.75%、64.58%)及对照组(20.83%、16.67%、18.75%、10.42%)(χ~2值分别为56.541、54.665、49.886、66.544,P值均<0.05);TB组椎间盘组织中MMP-9、MMP-13、TIMP-1、TIMP-3、表达水平均明显高于ID组及对照组(F值分别为12.451、37.423、17.483、9.167,P值均<0.05),MMP-9/TIMP-1、MMP-13/TIMP-3比值升高。结论:MMP-9、MMP-13、TIMP-1、TIMP-3在脊柱结核椎间盘组织中均呈高表达,导致MMP-9/TIMP-1、MMP-13/TIMP-3比例失衡,表明基质金属蛋白酶(MMPs)和基质金属蛋白酶抑制剂(TIMPs)可能均参与了脊柱结核椎间盘的破坏。均与脊柱结核椎间盘破坏。
Objective： To investigate the expression and significance of matrix metalloproteinase MMP-9,MMP-13 and their inhibitors TIMP-1,TIMP-3 in spinal tuberculosis intervertebral disc tissue. Methods：48 cases of patients with spinal tuberculosis treated by operation from January 2012 to January 2016 in our hospital were selected as TB group,another 48 cases with disc herniation treated by operation were selected as ID group,and patients with traumatic spinal fractures treated in our hospital in the same period were selected as the control group. Then the positive rate and the level of expression in the three groups were detected by immunohistochemistry assay and real-time quantitative PCR（ RT-PCR） technique. Results： The positive rate of MMP-9 was 93.75%,MMP-13 positive rate was 89.58%,TIMP-1 positive rate was 87.50%,TIMP-3 positive rate was 91.67% in TB group,which were all significantly higher than those in ID group（ 70.83%,66.67%,68.75%,64.58%） and the control group（ 20.83%,16.67%,18.75%,10.42%）（ X2 = 56.541,54.665,49.886 and 66. 544,respectively,P 〈 0. 05）; The expression levels of MMP-9,MMP-13,TIMP-1,TIMP-3TB in intervertebral disc tissue group were significantly higher than those in ID group and the control group（ F = 12.451,37.423,17.483 and 9. 167,respectively,P 〈 0. 05）,the ratio of MMP-9/TIMP-1 and MMP-13/TIMP-3 all increased. Conclusion： MMP-9,MMP-13,TIMP-1,TIMP-3 are highly expressed in spinal tuberculosis disc tissue,which result in the imbalance of MMP-9/TIMP-1,MMP-13/TIMP-3,indicating that the matrix metal proteases（ MMPs） and matrix metalloproteinase inhibitors（ TIMPs） may participate in the damages of spinal tuberculosis intervertebral disc.
出处
《河北医学》
CAS
2017年第3期353-356,共4页
Hebei Medicine
基金
新疆维吾尔自治区自然科学基金
(编号:2016D01C224)
关键词
基质金属蛋白酶
基质金属蛋白酶抑制剂
脊柱结核
椎间盘
Matrix metalloproteinase
Matrix metalloproteinase inhibitor
Spinal tuberculosis
Intervertebral disc
