摘要
目的 探讨Notch1基因在妊娠期高血压（妊高症）、重度子痫前期及正常胎盘组织中的表达水平及其相关的临床意义。方法分别采用免疫组化和荧光实时定量PCR检测50例妊高症、30例重度子痫前期及50例正常胎盘组织中Notchl基因的表达情况。结果免疫组化检测Nocth1蛋白在妊高症组、重度子痫组及正常对照组中的表达率分别为52．0％、43．4％、76％，组间比较差异均有统计学意义（P〈0．05）；荧光实时定量PCR检测Notchl基INmRNA含量在妊高症组、重度子痫组及正常对照组中分别为（4．10±0．57）、（3．07±0．45），（4．88±0．70），组间比较差异均有统计学意义（P〈0．05）。结论Notch1基因的低表达与妊娠期高血压的发生、发展存在相关性。
Objective To study the expression of Notchl gene in hypertensive disorder complicating pregnancy （ pregnancy-induced hypertension ） , severe preeclampsia and normal placenta and its clinical significance. Methods The expression of Notchl gene in 50 cases of pregnancy-induced hypertension, 30 cases of pre-eclampsia and 50 cases of normal placenta were detected by immunohistochemistry and real-time quantitative PCR. Results The expression of Nocthl protein in PIH group, severed eclampsia group and normal control group were 52.0%, 43.4% and 76% respectively. There was significant difference between the two groups （P〈0.05） .The mRNA levels of Notchl mRNA in PIH group, severe eclampsia group and normal control group were 4.10 ±0.57, 3.07 ± 0.45 and 4.88 ± 0.70 respectively. There was significant difference between the two groups （ P〈0.05 ） . Conclusion The low expression ofNotchl mRNA is associated with the development of PIH.
出处
《浙江临床医学》
2017年第5期804-806,共3页
Zhejiang Clinical Medical Journal
基金
浙江省人口及计划生育项目（浙人口计生委[2011]50号）
