高危型HPV感染与宫颈上皮病变组织中MEKK3、NF-κB基因表达的关系被引量：6

Relationship of high-risk HPV infection with MEKK3 and NF-κB expression in cervical intraepithelial neoplasia tissue

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摘要目的:研究宫颈上皮病变组织中高危型HPV感染与MEKK3、NF-κB表达的关系。方法:收集2013年5月~2016年3月期间125例宫颈活检标本进行研究,宫颈炎症标本、宫颈上皮内瘤变标本、宫颈癌标本分别纳入炎症组、CIN组、恶性组,采用HPV-DNA分型检测试剂盒测定HPV分型,采用免疫组化试剂盒测定MEKK3、NF-κB的蛋白表达量,采用荧光定量PCR试剂盒测定MEKK3、NF-κB以及下游分子的mRNA表达量。结果:高危型HPV阳性宫颈组织中MEKK3、NF-κB的蛋白阳性表达率均显著高于高危型HPV阴性宫颈组织,高危型HPV阳性宫颈组织中MEKK3、NF-κB、Bcl-2、XIAP、Bmi-1、TGF-β、Vimentin的mRNA表达量均显著高于高危型HPV阴性宫颈组织;MEKK3和NF-κB阳性表达组织中Bcl-2、XIAP、Bmi-1、TGF-β、Vimentin的mRNA表达量均显著高于MEKK3和NF-κB阴性表达组织。结论:高危型HPV感染会通过MEKK3/NF-κB通路来增加宫颈上皮病变组织中增殖基因Bcl-2、XIAP、Bmi-1以及侵袭基因TGF-β、Vimentin的表达量。 Objective： To study the relationship of high-risk HPV infection with MEKK3 and NF-κB expression in cervical intraepithelial neoplasia tissue.Methods： A total of 125 cases of cervical biopsy specimens between May 2013 and March 2016 were collected, the cervical inflammation specimens, cervical intraepithelial neoplasia specimens and cervical cancer specimens were included in inflammation group, CIN group and malignant group respectively, HPV-DNA typing detection kits were used to determine HPV typing, immunohistochemical kits were used to determine MEKK3 and NF-κB protein expression, and fluorescent quantitative PCR kits were used to determine the mRNA expression of MEKK3, NF-κB and downstream molecules.Results： MEKK3 and NF-κB protein expression in high-risk HPV-positive cervical tissue were significantly higher than those in high-risk HPV-negative cervical tissue, and MEKK3, NF-κB, Bcl-2, XIAP, Bmi-1, TGF-β and Vimentin mRNA expression in high-risk type HPV-positive cervical tissue were significantly higher than those in high-risk HPV-negative cervical tissue;Bcl-2, XIAP, Bmi-1, TGF-β and Vimentin mRNA expression in tissue with positive MEKK3 and NF-κB expression were significantly higher than those in tissue with negative MEKK3 and NF-κB expression.Conclusions： High-risk HPV infection will increase the expression of proliferation genes Bcl-2, XIAP and Bmi-1 as well as invasion genes TGF-β and Vimentin in cervical intraepithelial neoplasia tissue through MEKK3/NF-κB pathway.

作者鲁燕飞陈国强陈锐

机构地区湖北省黄冈市中心医院检验科

出处《海南医学院学报》 CAS 2017年第5期589-592,共4页 Journal of Hainan Medical University

基金长江大学黄冈临床医学院(WJ2016-YZ-44)~~

关键词宫颈上皮内瘤变高危型HPV 有丝裂原活化蛋白激酶/细胞外信号调节激酶3 核转录因子ΚB Cervical intraepithelial neoplasia High-risk HPV Mitogen activated protein kinase/ extracellular signalregulated kinase 3 Nuclear factor κB

分类号 R737.33 [医药卫生—肿瘤]

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参考文献2

1蒋燕明,李力.宫颈上皮内瘤变进展相关基因的生物信息学分析[J].中国肿瘤临床,2016,43(19):840-844. 被引量：14
2刘平,张红莉.宫颈上皮内瘤变组织中MEKK3、NF-κB的表达量及其与高危型HPV感染的相关性研究[J].海南医学院学报,2016,22(19):2247-2250. 被引量：10

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