摘要
目的探讨妊娠期糖尿病(GDM)母亲分娩新生儿与健康足月新生儿脐血血清S100B蛋白、caspase-3蛋白含量变化及其与新生儿行为神经测定(NBNA)评分的关系,并分析S100B蛋白、caspase-3蛋白含量与GDM母亲分娩新生儿脑损伤的关系。方法选择2015年1月至2016年3月于连云港市第一人民医院产科分娩的86例足月新生儿为研究对象。根据母亲孕期是否合并GDM,将其分为GDM组(n=33)和对照组(n=53)。采用酶联免疫吸附试验(ELISA)法测定2组新生儿脐血血清S100B蛋白、caspase-3蛋白含量,并于生后第3天对新生儿进行NBNA评分。结果 (1)2组新生儿性别构成比、胎龄、出生体重、母亲年龄等一般临床资料比较,差异均无统计学意义(P>0.05)。(2)GDM组新生儿脐血血清S100B蛋白、caspase-3蛋白含量均高于对照组[(38.8±9.3)pg/mL vs(12.4±2.6)pg/mL,(5.3±1.7)μg/mL vs(2.8±0.6)μg/mL],并且差异均有统计学意义(t=8.314、15.977,P<0.001)。GDM组NBNA评分低于对照组[(36.3±1.2)分vs(38.3±1.2)分],并且差异亦有统计学意义(t=8.129,P<0.001)。(3)GDM组中,新生儿脐血血清S100B蛋白、caspase-3蛋白含量与NBNA评分呈负相关关系(r=-0.583、-0.814,P<0.001),新生儿脐血血清S100B蛋白含量与caspase-3蛋白含量呈正相关关系(r=0.394,P=0.023)。对照组中,新生儿脐血血清S100B蛋白、caspase-3蛋白含量与NBNA评分,均无相关性(P>0.05)。结论 GDM母亲分娩新生儿可能存在脑损伤,新生儿脐血血清S100B蛋白和caspase-3蛋白可作为预判GDM母亲分娩新生儿脑损伤及其程度的指标。
ObjectiveTo discuss correlation on the S100B protein, caspase-3 protein levels of neonatal umbilical cord blood and neonatal behavior neurological assessment （NBNA） score in gestational diabetes mellitus （GDM） women.
MethodsFrom January 2015 to March 2016, a total of 86 neonates delivered in the First People′s Hospital of Lianyungang were recruited into this study. They were divided into GDM group （n=33） and control group （n=53） on the basis of mother with or without GDM during pregnancy period. The content of S100B protein and caspase-3 protein of neonatal umbilical cord blood were detected by enzyme-linked immuosorbent assay （ELISA）. The NBNA scores were determined at postnatal day 3.Results①There were no significant differences between two groups in the aspects of constitution ratio of gender, gestational age, birth weight maternal age, etc. （P〉0.05）. ②The contents of S100B protein and caspase-3 protein in GDM group were higher than those of control group [（38.8±9.3） pg/mL vs （12.4±2.6） pg/mL, （5.3±1.7） μg/mL vs （2.8±0.6） μg/mL], and the differences were statistically significant （t=8.314, 15.977; P〈0.001）. The NBNA score in GDM group was lower than that of control group [（36.3±1.2） scores vs （38.3±1.2） scores], and the difference was statistically significant （t=8.129, P〈0.001）. ③In GDM group, the content of S100B protein and caspase-3 protein of neonatal umbilical cord blood were negative correlated with NBNA score in GDM group, respectively （r=-0.583, -0.814; P〈0.001）, and the content of S100B protein of neonatal umbilical cord blood was positive correlated with caspase-3 protein （r= 0.394, P=0.023 ）. While in control group, S100B protein, caspase-3 protein of neonatal umbilical cord blood and NBNA score had no correlation with each other （P〉0.05）.ConclusionsGDM is probably the one of the risk factors of neonatal brain damage. It is suggested that S100B protein and caspase-3 protein of neonatal umbilical cord blood can be used as an reference index predicting the degree of brain damage.
出处
《中华妇幼临床医学杂志（电子版）》
CAS
2017年第2期189-193,共5页
Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition)
基金
江苏省妇幼健康科研项目(F201553)
连云港市卫生科技项目(201507)~~
