摘要
目的利用DNA条形码技术对采自我国22个省的姬鼠属样本进行鉴定,调查其分类及在我国的分布情况,为我国鼠传疾病的防控与监测提供数据支持。方法对鼠类样本的线粒体细胞色素C氧化酶亚基Ⅰ(COⅠ)基因片段进行扩增并测序,构建系统发育树,并与Gen Bank中相关序列进行比对。结果 119份姬鼠属样本经PCR检测均获得有效COⅠ基因扩增片段,通过构建分子进化树得到8个分支,与Gen Bank中的数据进行比对,可确定为8个独立的鼠种,分别为黑线姬鼠、齐氏姬鼠、朝鲜姬鼠、大耳姬鼠、澜沧江姬鼠、中华姬鼠、乌拉尔姬鼠及1个姬鼠属未定种。结论通过对鼠类样本的准确鉴定,可确定这些种在我国较为准确的分布情况,修订了原基于形态鉴定的物种分布。
Objective To investigate the taxonomy and distribution of Apodemus species in China, and to provide supportive data for prevention and control of rodent borne disease. Methods DNA barcoding was employed to identify Apodemus samples collected from 22 provincial areas in China. Genomic DNA was extracted from tissue samples for PCR amplification of the mitochondrial cytoehrome C oxidase subnnit I （CO I ） gene, followed by sequencing. The obtained sequences were compared with the related sequences in GenBank. Then a phylogenetic tree was constructed. Results CO I gene fragment were amplified from 119 Apodemus samples, and they were sorted into eight branches through phylogenetic reconstruction. Compared with the data in the GenBank, the collected samples were identified as eight independent species： Ap. agrarius, Ap. chevrieri, Ap. peninsulae, Ap. latronum, Ap. ilex, Ap. draco, Ap. uralensis and an undefined species. Conclusion The accurate distribution of Apodemus species in China can be determined by effective identification of samples through DNA barcoding. In addition, the results revised the Apodemus species distribution based on the morphological identification.
出处
《中国媒介生物学及控制杂志》
CAS
2017年第2期97-103,共7页
Chinese Journal of Vector Biology and Control
基金
国家科技重大专项(2012ZX10004-219)~~
关键词
DNA条形码
姬鼠属
种类鉴定
分布
DNA barcoding
Apodemus
Species identification
Distribution
