摘要
目的探讨富氢盐水对高压电烧伤大鼠缺血再灌注损伤指标、白细胞介素-8(IL-8)以及肿瘤坏死因子-α(TNF-α)水平的影响。方法选取SPF级雄性大鼠60只,采用随机数字表法分为四组,即使用生理盐水进行腹腔注射的高压电烧伤实验组、腹腔注射富氢盐水治疗高压电烧伤的治疗组、单纯腹腔注射生理盐且无高压电烧伤的空白对照组,以及腹腔注射罂粟碱治疗高压电烧伤的阳性对照组,每组15只。治疗后,检测血浆中6-酮-前列腺素F1α(6-Keto-PGF1α)、血栓素B2(TXB2)水平,并检测电烧伤组织黄嘌呤氧化酶(XOD)、丙二醛(MDA)、超氧化物歧化酶(SOD)、TNF-α及IL-8水平。结果治疗组大鼠血浆6-keto-PGF1α和TXB2水平显著高于空白对照组(P<0.01),显著低于实验组和阳性对照组(P<0.01)。治疗组大鼠组织SOD水平显著低于空白对照组(P<0.01),显著高于实验组和阳性对照组(P<0.01);治疗组大鼠组织XOD及MDA水平显著高于空白对照组(P<0.01),显著低于实验组和阳性对照组(P<0.01)。治疗组大鼠组织TNF-α及IL-8水平显著高于空白对照组(P<0.01),显著低于实验组和阳性对照组(P<0.01)。结论富氢盐水治疗高压电烧伤大鼠可有效保护受损区,最大限度地降低缺血再灌注损伤,且有效抑制细胞氧化应激,控制炎性因子表达可能是富氢盐水发挥生物学功能的主要机制。
Objective To investigate influence of hydrogen rich saline on ischemia reperfusion injury indexes, IL-8, TNF-α levels in high voltage burn rats. Methods 60 male SPF rats were selected and divided into four groups by ran- dora number table method. There were intraperitoneal injection of saline as high-voltage burn experimental group, intraperitoneal injection of hydrogen rich saline as high voltage burn treatment group, injection of physiological saline without high voltage burn as blank control group, intraperitoneal injection of papaverine with high-voltage burn as pos- itive control group. Each group had 15 rats. After treatment, plasma 6-Keto-PGF1α, TXB2 levels were detected. XOD, M DA, SOD, TNF-α and IL-8 levels of high voltage burn tissues were detected. Results Plasma 6-Keto-PGF1α, TXB2 levels in treatment group were significantly higher than those in blank control group （P 〈 0.01）, which were significantly lower than those in experimental group and positive control group （P 〈 0.01）. SOD level in treatment group was significantly lower than that in blank control group （P 〈 0.01）, which were significantly higher than that in experimental group and positive control group （P 〈 0.01）. XOD, MDA levels in treatment group were significantly higher than those in blank control group （P 〈 0.01）, which were significantly lower than those in experimental group and positive control group （P 〈 0.01）. TNF-α, IL-8 levels in treatment group were significantly higher than those in blank control group （P 〈 0.01）, which were significantly lower than those in experimental group and positive control group （P 〈 0.01）. Conclusion Hydrogen rich saline in the treatment of high voltage burn rats can effectively protect the damaged area and maximum degree of re-duction ischemia reperfusion injury, which can also effective inhibition of cellular oxidative stress and control of inflammatory factor expression. Which may be the main mechanism of hydrogen rich saline.
出处
《中国医药导报》
CAS
2017年第10期22-25,30,共5页
China Medical Herald
基金
河北省医学科学研究重点课题计划项目(20150197)
