摘要
目的采用牛角膜混浊和渗透性试验(bovine corneal opacity and permeability,BCOP)方法对滴眼液的角膜渗透性进行评价。方法利用BCOP法,以无酚红MEM培养液为介质,前室加滴眼液样品,于不同时间点后室取样,用紫外-可见分光光度法测定其苄达赖氨酸的含量,比较不同滴眼液样品的角膜渗透性。结果不同厂家样品表现出不同的角膜渗透性,包括角膜累积渗透量、透过速率、表观渗透系数和稳态渗透速率均不同。结论可用BCOP法进行眼用制剂的角膜渗透性研究。
OBJECTIVE To evaluate corneal permeability of eye drops by bovine corneal opacity and permeability（BCOP） assay. METHODS Used BCOP method, phenol red-free MEM medium was used as release medium, loaded eye drops into the anterior chamber, collected the samples form posterior chamber in different time point and detected the content of bendazac lysine by UV spectrophotography to compare corneal permeability of different eye drops. RESULTS Eye drops from different manufacturers showed different corneal permeability, including the outcomes of permeation amount, permeation rate, apparent permeability and steady-state rate of permeation had been verified. CONCLUSION The BCOP assay can be used in the study of corneal permeability of ophthalmic preparation.
出处
《中国现代应用药学》
CAS
CSCD
2017年第3期390-392,共3页
Chinese Journal of Modern Applied Pharmacy
