摘要
目的探讨关节软骨破坏与一氧化氮/诱导型一氧化氮合成酶(NO/i NOS)动态变化关系.方法40只健康成年Wistar雄性大鼠随机分为对照组(自由活动)和实验组(过度运动训练).于0、1、2、3、6周时采集大鼠全膝关节,4%PFA液固定、石蜡包埋、切片、染色;通过Safranin-O染色观察软骨形态学变化,ELISA法测定血清/关节液中NO/i NOS含量变化,Mankin's评分,统计并分析软骨病理学改变.结果过度运动训练能导致关节软骨从细胞排序紊乱、基质丢失到表面毛糙、裂缝等系列软骨破坏和退变病理改变过程;过度运动训练能使大鼠血清、关节液中NO/i NOS的含量增加,血清NO/i NOS组:1周(48.088±1.534),(3.058±0.073);2周(54.574±0.800),(6.630±0.524);3周(68.020±3.184),(7.384±0.306);6周(104.394±8.094),(11.364±0.882).关节液NO/i NOS组:1周(65.814±1.610),(8.126±0.724);2周为(72.766±2.344),(9.694±0.564);3周(88.816±0.819),(11.326±1.473);6周为(120.204±12.044),(15.166±1.198),并随运动量递增,保持较高浓度水平.结论关节液或血清中NO/i NOS含量变化与关节软骨的破坏程度呈正向关系.
Objective To investigate the dynamic changes of articular cartilage destruction and nitric oxide nitric oxide/inducible nitric oxide synthase （NO/iNOS） . Methods Forty healthy adult male Wistar rats were randomly divided into control group （free movement） and experimental group （overtraining） . Total knee joints of rat were collected at week 0, 1, 2, 3, and 6, fixed with 4% PFA, embedded in paraffin, sectioned and stained. Cartilage morphological changes were observed by Safranin-O staining, NO/iNOS content changes in serum and synovial fluid were determined by ELISA, Mankin＇s scores were obtained, and cartilage pathological changes were analyzed. Results Overtraining resulted in cell sorting disorders, loss of matrix, destruction like rough surface and cracks, and degeneration of cartilage pathology process. Levels of NO/iNOS in serum and synovial fluidincreased after overtraining：week 1 （48.088 ± 1.534）, （3.058 ± 0.073） ; week 2 （54.574 ± 0.800）, （6.630 ± 0.524） ; week 3 （68.020 ± 3.184）, （7.384 ± 0.306） ; weeks 6 （104.394 ± 8.094）, （11.364 ± 0.882） Levels of NO/iNOS in synovial fluid group were： week 1 （65.814 ±1.610） , （8.126 ±0.724） ; week 2 （72.766 ± 2.344）, （9.694 ± 0.564） ; week 3 （88.816 ± 0.819）, （11.326 ± 1.473） ; week 6 （120.204 ± 12.044） , （15.166 ± 1.198） , and high level of concentration was found with the increasing amount of exercise. Conclusion There is a positive correlation between the levels of NO/iNOS in synovial fluid or serum and the extent of the damage of articular cartilage.
作者
唐涛
孙先润
姜艳萍
张敏
马骅
龙宇
TANGTao SUN Xian-mn JIANG Yan-ping ZHANG Min MA Hua LONG Yu(Dept. of Rehabilitation, The 1st People＇s Hospital of Yunnan Province, Kunming Yunnan 650032 The Medical School of Kunming University of Science and Technology, Kunming Yunnan 650500 Yunnan University, Kunming Yunnan 650091 School Hospital of Kunming Medical University, Kunming Yunnan 650009, China)
出处
《昆明医科大学学报》
CAS
2016年第11期28-32,共5页
Journal of Kunming Medical University
基金
云南省应用基础研究(昆医联合专项)基金资助项目(2015FB095)
云南省应用基础研究计划项目(2013FZ182)
