摘要
目的设计一种能快速简便测定尿碘的尿碘测定仪。方法以砷铈催化动力曲线法为本仪器测定方法,尿样按WS/T 107.1—2016方法消化,在420 nm下以1 s的频率扫描100 s记录催化动力曲线,以log A-t曲线的斜率来定量。设计了新型尿碘测定仪的结构光学系统及软件界面。结果本尿碘测定仪在尿碘浓度为0μg/L^1 000μg/L时线性关系良好;方法的检出限为12μg/L;考察高、中、低3个浓度的加标样品,加标回收率在93.9%~108.7%,相对标准差(RSD)为0.32%~2.04%;对20份尿样分别采用催化动力曲线法和WS/T 107.1—2016 2种方法测定其碘含量,测定结果差异无统计学意义(P>0.05)。结论此尿碘测定仪测定尿碘准确、快速,样品消化后2 min内可完成分析,适合用于尿碘的快速测定。
Objective To design a new type of urinary iodine detector which can measure urinary iodine fast and accurately. Methods The principle of this new instrument was based on the catalytic kinetic curve of the Sandell -Kolthoff reaction. According to WS/T 107. 1--2016, urine was digested. The kinetic curve was recorded about 100 s by the frequency of 1 s at 420 nm, and the urinary iodine was quantified according to the slope of the logA - t curve. The structural optical system and software interface of a new type of urinary iodine analyzer were designed. Results Good linearity was obtained when the concentration of urine idiom was within 0 μg/L - 1 000 μg/L, and the detection limit of this method was 12 μg/L. The average re- covery of urinary iodine was within 93.9% - 108.7% at three spiked levels. The reletive standard deviations（RSDs） were with- in 0.32% -2.04%. 20 urine samples were determined by both catalytic kinetic curve and WS/T 107.1--2016 method, and there was no statistical significance in the difference between the two methods （ P 〉 0.05 ）. Conclusion This urinary iodine detector can measure urinary iodine fast and accurately. Analysis can be completed within 2 min after sample digestion. This urinary iodine detector was proved suitable for the fast determination of urinary iodine.
出处
《中国卫生检验杂志》
CAS
2017年第7期954-957,共4页
Chinese Journal of Health Laboratory Technology
基金
四川大学华西公共卫生学院卫生检验教研室
杭州市职业病防治院的大力支持和帮助
关键词
尿碘
尿碘测定仪
催化动力曲线
Urinary iodine
Urinary iodine detector
Catalytic kinetic curve
