摘要
目的探讨常规手淫取精、经皮附睾穿刺抽吸术、睾丸活检术三种不同方式获得新鲜精子行卵胞浆内单精子注射(intracytoplasmic sperm injection ICSI)助孕结局。方法回顾性分析2014年1月-2016年6月我科室采用ICSI助孕的103新鲜周期。将患者分为ABC三组(A组:45例严重少弱精子症患者常规手淫取精获得精子助孕、B组:23例梗阻性无精症患者经皮附睾穿刺抽吸术获得附睾精子助孕、C组:35例无精子症患者经睾丸活检获得睾丸精子助孕)女方均为年龄在25岁到37岁生育力筛查正常或单纯因输卵管因素导致的不育。比较三组的2pn受精率、优质胚胎率、着床率、临床妊娠率。结果 A组、B组、C组2pn受精率、优质胚胎率、着床率、临床妊娠率为72.91%VS 76.91%VS 71.91%;42.55%VS 51.55%VS 48.55%;32.14%VS 38.14%VS 35.27%;53.33%VS 56.52%VS 54.29%(P>0.05)。结论不同来源的精子行ICSI助孕后结局相近,来源不同精子可能不是影响ICSI妊娠结局的主要因素。
Objective To investigate the therapeutic outcome of intracytoplasmic sperm injection（ICSI） by the common masturbation sperm,percutaneous epididymal sperm aspiration and testicular biopsy. Methods The data of 103 patients received ICSI from January 2014 to June 2016 in our laboratory was retrospectively analyzed. The patients were divided into three groups of ABC（Group A：45 patients with severe oligoasthenozoospermia whose sperm were collected by conventional masturbation for sperm fertility. Group B：23 patients with obstructive azoospermia whose sperm were collected by percutaneous epididymal sperm aspiration.Group C：35 patients with azoospermia whose sperm were collected by testicular biopsy. The women aged 25 to 37 adopting fertility screening showed normal or infertility simply caused by tubal factor. A comparison of three groups of 2pn fertilization rate,high quality embryo rate,implantation rate and clinical pregnancy rate were conducted. Results Group A,group B and group C of 2pn fertilization rate,high quality embryo rate,implantation rate and clinical pregnancy rate were 72.91% VS 76.91% VS 71.91%;42.55% VS 51.55% VS 48.55%;32.14% VS 38.14% VS 35.27%;53.33% VS 56.52% VS 54.29%（P0.05） respectively. Conclusions The therapeutic outcomes of ICSI with different sperm sources are similar,the sperm from different sources may not be the main factor affecting the outcome of ICSI pregnancy.
出处
《实验与检验医学》
CAS
2017年第2期190-192,共3页
Experimental and Laboratory Medicine
基金
江西省卫生厅科技计划(20143059)
