摘要
目的评价囊虫IgG抗体检测试剂盒的诊断效果,为临床应用提供参考。方法按照囊虫IgG抗体检测试剂盒说明书,采用ELISA法检测囊虫病、包虫病、带绦虫病及健康人血清。结果在30例活动期脑囊虫病病人中,28例呈囊虫IgG抗体阳性,敏感性为93.33%。100份健康人血清均无阳性反应。在42例带绦虫感染者,2例囊虫抗体阳性,阳性率为4.76%。在60份包虫病血清中,阳性41份,该试剂盒与包虫病的交叉反应率为68.33%。结论所评价试剂盒敏感性较好,但特异性差,与包虫病的交叉反应率高。以上提示国内囊虫病免疫学诊断试剂的质量有待提高。
Objective To evaluate the ELISA kit for detection of IgG antibodies against Taenia solium cysticercus in hu-mans,so as to provide a reference for its application in clinical practice. Methods The sera collected from the patients with neurocysticercosis,echinococcosis,taeniasis and healthy people,respectively,were checked by ELISA for specific IgG anti-bodies against T. solium cysticercus,as described in the instruction of the kit. Results Of the 30 patients with neurocysticerco-sis at active stage,28 showed seropositive reaction,indicating a sensitivity of 93.33%. There were no positive reactions in the 100 healthy people. Two of the 42 persons with taeniasis were seropositive with an infection rate of 4.76%. Forty-one of the 60 persons with echinococcosis showed positive reactions,suggesting that the cross reaction rate of the kit with echinococcosis was 68.33%. Conclusions The assessed ELISA kit has a reasonably high sensitivity but a poor specificity,and the cross reaction rate with echinococcosis is very high. It is urgent to improve the sensitivity and specificity of the diagnostic kit for detection of T. solium cysticercosis in China.
出处
《中国血吸虫病防治杂志》
CAS
CSCD
北大核心
2017年第2期228-230,共3页
Chinese Journal of Schistosomiasis Control
基金
四川省科技厅重大项目(2014SZ0151)
