摘要
目的建立地桃花提取物中杨梅苷、异槲皮苷和木犀草素的高效液相色谱法(HPLC)同时测定方法。方法采用HPLC法,Kromasiol-C18(250 mm×4.6 mm,5μm),乙腈-0.25%醋酸水(31∶69)等度洗脱,检测波长:351 nm,流速:1 m L·min-1;柱温30℃。结果杨梅苷、异槲皮苷和木犀草素分别在6.97~278.80 mg·L^(-1)、7.29~291.50 mg·L^(-1)和2.96~118.40 mg·L^(-1)范围内线性关系良好,平均回收率分别为98.39%、98.53%和97.41%。结论该方法准确、可靠,重复性好,可用于地桃花提取物的质量评价与控制标准。
Objective To establish a HPLC method for simultaneous determination of the content of myricitrin,isoquercitrin and luteolin in extract of Urena lobata L. Methods The HPLC analysis was carried out on Kromasiol-C18column(4. 6 mm × 250 mm,5 μm) with acetonitrile-0. 25% acetic acid solution(31: 69) as the mobile phase for isocratic elution at a flow rate of 1 m L·min-1. The detection wavelength was 351 nm and the column temperature was 30 ℃. Results Good linear relationship were found in the the range of 6. 97-278. 80 mg·L^(-1)for myricitrin,7. 29-291. 50 mg·L^(-1)for isoquercitrin,2. 96-118. 40 mg·L^(-1)for luteolin. Their average recoveries were 98. 39%,98. 53% and 97. 41%,respectively. Conclusion The method is accurate,reliable,and can be used for the quality control and evaluation of extract of Urena lobata L.
出处
《安徽医药》
CAS
2017年第3期444-447,共4页
Anhui Medical and Pharmaceutical Journal
关键词
色谱法
高压液相
地桃花提取物
木犀草素
杨梅苷
异槲皮苷
Chromatography,high pressure liquid
Extract of Urena lobata L.
Luteolin
Myricitrin
Isoquercitrin
