摘要
目的探讨IL-33在胃癌组织中的表达变化及其意义。方法选择98例胃癌患者的癌组织、癌旁组织。患者中,早期胃癌16例,进展期胃癌82例;淋巴结转移76例、无淋巴结转移22例;另取同期36例健康体检者胃组织标本作为对照。应用免疫组化SP法和Real-time PCR法检测胃癌组织和正常胃组织中IL-33及其mRNA的表达情况,分析其与患者临床病理特征的关系,采用Kaplan-Meier法绘制IL-33表达阳性与阴性患者生存曲线,比较其中位生存时间。结果胃癌组织中IL-33表达阳性率高于正常胃组织(P均<0.05)。早期胃癌以及淋巴结转移阴性胃癌组织与正常胃组织IL-33表达阳性率比较,P均>0.05,而进展期胃癌以及有淋巴结转移胃癌组织与正常胃组织比较,P均<0.05。胃癌组织中IL-33 mRNA相对表达量高于正常胃组织(P<0.05);有无淋巴结转移胃癌组织相比,P<0.05。IL-33表达与胃癌患者性别、年龄、肿瘤生长部位及肿瘤大小无关(P均>0.05),但与肿瘤分化程度、组织浸润程度、淋巴结转移、远处转移以及临床分期有关(P均<0.05)。IL-33表达阴性患者中位生存时间为30.6个月,IL-33表达阳性患者中位生存时间为22.7个月,两者比较,P=0.001。结论胃癌组织中IL-33水平升高,其可能参与了胃癌的发生发展,并提示预后不良。
To investigate the expression of IL-33 in gastric carcinoma tissues and its significance.Methods Cancer tissues and adjacent tissues of 98 patients with gastric cancer were selected, among whom, there were 16 cases with early gastric cancer, 82 cases with advanced gastric cancer, 76 cases with lymph node metastasis and 22 cases without lymph node metastasis.Meanwhile, we selected 36 cases of normal gastric tissues from healthy people as the control group.Immunohistochemical S-P method and real-time PCR were applied to detect the expression of IL-33 and its mRNA in gastric carcinoma tissues as well as in normal gastric tissues.We analyzed the relationship between the expression of IL-33 and clinicopathological features of gastric carcinoma tissues.Survival curves between IL-33 expression positive and negative patients were made using the Kaplan Meier method, and the median survival time was compared.Results The positive rate of IL-33 expression in gastric cancer tissues was higher than that in normal gastric tissues （P〈0.05）.No significant difference was found in the positive rate of IL-33 expression between in early gastric cancer and lymph node metastasis-negative gastric cancer tissues and in the normal gastric tissues （all P〉0.05）, while significant difference was found between in advanced gastric cancer and lymph node metastasis-positive gastric cancer tissues and in the normal gastric tissues （all P〈0.05）.The relative expression of IL-33 mRNA in gastric cancer tissues was higher than that in the normal gastric tissues （P〈0.05）.After the comparison of those between gastric cancer tissues with and without lymph node metastasis, significant difference was found （P〈0.05）.The expression of IL-33 was not related with gender, age, tumor growth and tumor size （all P〉0.05）, but it was correlated with tumor differentiation, tissue infiltration, lymph node metastasis, distant metastasis and clinical stage （all P〈0.05）.The median survival time was 30.6 months in IL-33 negative patients and 22.7 months in patients with positive IL-33 expression, P=0.001.Conclusion The increment on the level of IL-33 in gastric cancer tissues indicates that IL-33 may be involved in the occurrence and development of gastric cancer and predict poor prognosis.
出处
《山东医药》
CAS
北大核心
2017年第13期5-8,共4页
Shandong Medical Journal
基金
国家自然科学基金面上项目(61371066)
江苏省医学重点人才资助项目(RC2011090)
江苏省重点研发计划(社会发展)(BE2015720)
江苏省六大高峰人才项目(WSW-041)
关键词
胃肿瘤
白细胞介素33
淋巴结转移
预后
stomach neoplasms
interleukin-33
lymph node metastasis
prognosis
