摘要
AIM：To investigate the effect of Htr A1 on the proliferation,migration and apoptosis of human retinal pigment epithelium（RPE） cells in the light injured model,as well as the expression of the apoptosis related molecules.METHODS：The human RPE cell line ARPE-19 was exposed to blue light to establish the light injured model.The cells were transfected with Htr A1 si RNA to knockdown Htr A1 expression.Subsequent expression of Htr A1 was determined by real-time polymerase chain reaction（RTPCR） and Western blot,respectively.Changes in cell proliferation,migration and apoptosis were assessed by cell counting kit-8（CCK-8）,Transwell assay and flow cytometry respectively,as well as changes in the m RNA and protein levels of Bax,Caspase-3 and Bcl-2 expression.RESULTS：Htr A1 was highly expressed in ARPE-19 cells after blue light irradiation.Knockdown of Htr A1 expression inhibited the proliferation,migration and apoptosis of the blue-light-irradiated ARPE-19 cells（P〈0.05）.Bax and Caspase-3 expression were significantly reduced both at m RNA and protein levels（P〈0.05） after si RNA treatment.Bcl-2 expression significantly increased in blue-lightirradiated ARPE-19 cells after si RNA interference（P〈0.05）.CONCLUSION：Silence of Htr A1 may inhibit the proliferation,migration and apoptosis of ARPE-19 cells in light injured model.Moreover,Htr A1 suppression in blue-lightirradiated ARPE-19 cells may ameliorate cell apoptosis through down-regulation of Bax and Caspase-3,and upregulation of Bcl-2 expression.
AIM：To investigate the effect of Htr A1 on the proliferation,migration and apoptosis of human retinal pigment epithelium（RPE） cells in the light injured model,as well as the expression of the apoptosis related molecules.METHODS：The human RPE cell line ARPE-19 was exposed to blue light to establish the light injured model.The cells were transfected with Htr A1 si RNA to knockdown Htr A1 expression.Subsequent expression of Htr A1 was determined by real-time polymerase chain reaction（RTPCR） and Western blot,respectively.Changes in cell proliferation,migration and apoptosis were assessed by cell counting kit-8（CCK-8）,Transwell assay and flow cytometry respectively,as well as changes in the m RNA and protein levels of Bax,Caspase-3 and Bcl-2 expression.RESULTS：Htr A1 was highly expressed in ARPE-19 cells after blue light irradiation.Knockdown of Htr A1 expression inhibited the proliferation,migration and apoptosis of the blue-light-irradiated ARPE-19 cells（P〈0.05）.Bax and Caspase-3 expression were significantly reduced both at m RNA and protein levels（P〈0.05） after si RNA treatment.Bcl-2 expression significantly increased in blue-lightirradiated ARPE-19 cells after si RNA interference（P〈0.05）.CONCLUSION：Silence of Htr A1 may inhibit the proliferation,migration and apoptosis of ARPE-19 cells in light injured model.Moreover,Htr A1 suppression in blue-lightirradiated ARPE-19 cells may ameliorate cell apoptosis through down-regulation of Bax and Caspase-3,and upregulation of Bcl-2 expression.
基金
Supported by the National Natural Science Foundation of China(No.81271025
No.81271023)
