期刊文献+

Intestinal organoid as an in vitro model in studying host-microbial interactions 被引量:1

Intestinal organoid as an in vitro model in studying host-microbial interactions
原文传递
导出
摘要 BACKGROUND: Organoid is an in vitro three-dimensional organ-bud that shows realistic microanatomy and physiological relevance. The progress in generating organoids that faithfully recapitulate human in vivo tissue composition has extended organoid applications from being just a basic research tool to a translational platform with a wide range of uses. Study of host- microbial interactions relies on model systems to mimic the in vivo infection. Researchers have developed various experimental models in vitro and in vivo to examine the dynamic host-microbial interactions. For some infectious pathogens, model systems are lacking whereas some of the used systems are far from optimal. OBJECTIVE: In the present work, we will review the brief history and recent findings using organoids for studying host- microbial interactions. METHODS: A systematic literature search was performed using the PubMed search engine. We also shared our data and research contribution to the field. RESULTS: we summarize the brief history of 3D organoids. We discuss the feasibility of using organoids in studying host- microbial interactions, focusing on the development of intestinal organoids and gastric organoids. We highlight the advantage and challenges of the new experimental models. Further, we discuss the future direction in using organoids in studying host- microbial interactions and its potential application in biomedical studies. CONCLUSION: In combination with genetic, transcriptome and proteomic profiling, both murine- and human-derived organoids have revealed crucial aspects of development, homeostasis and diseases. Specifically, human organoids from susceptible host will be used to test their responses to pathogens, probiotics, and drugs. Organoid system is an exciting tool for studying infectious disease, microbiome, and therapy. BACKGROUND: Organoid is an in vitro three-dimensional organ-bud that shows realistic microanatomy and physiological relevance. The progress in generating organoids that faithfully recapitulate human in vivo tissue composition has extended organoid applications from being just a basic research tool to a translational platform with a wide range of uses. Study of host- microbial interactions relies on model systems to mimic the in vivo infection. Researchers have developed various experimental models in vitro and in vivo to examine the dynamic host-microbial interactions. For some infectious pathogens, model systems are lacking whereas some of the used systems are far from optimal. OBJECTIVE: In the present work, we will review the brief history and recent findings using organoids for studying host- microbial interactions. METHODS: A systematic literature search was performed using the PubMed search engine. We also shared our data and research contribution to the field. RESULTS: we summarize the brief history of 3D organoids. We discuss the feasibility of using organoids in studying host- microbial interactions, focusing on the development of intestinal organoids and gastric organoids. We highlight the advantage and challenges of the new experimental models. Further, we discuss the future direction in using organoids in studying host- microbial interactions and its potential application in biomedical studies. CONCLUSION: In combination with genetic, transcriptome and proteomic profiling, both murine- and human-derived organoids have revealed crucial aspects of development, homeostasis and diseases. Specifically, human organoids from susceptible host will be used to test their responses to pathogens, probiotics, and drugs. Organoid system is an exciting tool for studying infectious disease, microbiome, and therapy.
作者 Jun Sun
出处 《Frontiers in Biology》 CAS CSCD 2017年第2期94-102,共9页 生物学前沿(英文版)
关键词 bacteria colonoids enteroids gastric organoids host-microbial interactions H. pylori inflammation intestinalorganoids microbiome ORGANOIDS tight junctions SALMONELLA stem-cell differentiation ZO-1 bacteria, colonoids, enteroids, gastric organoids, host-microbial interactions, H. pylori, inflammation, intestinalorganoids, microbiome, organoids, tight junctions, Salmonella, stem-cell differentiation, ZO-1
  • 相关文献

同被引文献1

引证文献1

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部