摘要
目的了解本地区近四年乙型肝炎患者HBV DNA阳性率与病毒载量分布特点。方法采用荧光定量聚合酶链反应(PCR)检测2012年至2015年间13 039例HBsAg阳性患者HBV DNA载量,并进一步分析24例HBeAg阳性、31例HBeAg阴性初诊乙肝患者4年间接受正规治疗后HBV DNA变化趋势。结果本地区近4年间血清HBV DNA阳性率依次为48.6%、46.18%、38.69%、37.66%,呈逐年下降趋势,差异具有统计学意义(P<0.01);各年度病毒载量均值的对数值分别为(5.48±1.56),(5.27±1.72),(5.29±1.68),(5.35±1.85)拷贝/mL。2013年、2014年、2015年分别与2012年相比,差异均有统计学意义(P<0.01、P<0.01、P<0.05),而低病毒载量(即病毒拷贝数的对数值小于5拷贝/mL)患者比例2012年明显大于其他年份,2012年分别与2013年、2014年、2015年相比,差异具有统计学意义(P<0.01,P<0.01,P<0.05);HBeAg阳性/阴性乙肝患者接受正规治疗后,其HBV DNA载量均呈逐年下降趋势,其中第一年下降显著(P<0.01)。结论近4年本地区血清HBV DNA阳性率呈逐年下降趋势,且低病毒载量比例明显增加。
Objective To investigate the positive rate of HBV DNA and viral load distribution among local patients with hepatitis B in the past 4 years. Methods The HBV DNA loads of 13 039 HBsAg positive pa- tients between 2012 and 2015 were detected with fluorescence quantitative PCR. The changes of HBV DNA of 24 HBeAg positive and 31 HBeAg negative hepatitis B patients who were newly diagnosed and given regu- lar treatment during the four years were further analyzed. Results The positive rates of serum HBV DNA in the past four years were 48. 6%, 46. 18%, 38. 69% and 67. 66%, decreasing year by year with significantly statistical difference （P〈0.01） . The logarithms of the mean annual viral load were （5.48±1.56）, （5. 27±1.72）, （5. 29±1.68） and （5. 35±1.85） eopies/mL for each year, among which significant differences existed between the value in 2012 and those in 201a, 2014 and 2015 （P〈0.01, P〈0. 01, P〈0. 05） . Meanwhile, the proportion of patients with low viral load （logarithm of viral loads〈5 copies/mL） was significantly higher in 2012 than tb.at in 2013, 2014 and 2015 respectively （P〈0.01, P〈0. 01, P〈0. 05） . The HBV DNA loads of HBeAg positive/ negative hepatitis B patients after receiving regular treatment showed a declining trend year by year and de- clined most significantly in the first year （P〈0.01） . Conclusion The positive rate of serum HBV DNA de- creased gradually and the proportion of low viral load increased significantly in the past 4 years in the local region.
出处
《中国微生态学杂志》
CAS
CSCD
2017年第4期434-437,共4页
Chinese Journal of Microecology
基金
国家自然科学基金资助项目(81672092)
