摘要
目的:运用液质联用技术研究重金属汞皮肤接触中华大蟾蜍后生理状态及泌酥中蟾蜍甾烯水平变化,为中药蟾酥品质评价提供参考。方法:配制不同质量浓度的氯化汞溶液(0.5,0.75,1.0 g·L^(-1))对蟾蜍进行半静水式处理,观察其生理状态,且在30 d后收集蟾蜍耳后腺分泌液,进一步采用液相-质谱联用技术测定蟾蜍泌酥中游离型蟾蜍甾烯的水平,结合Hem I热图软件进行数据分析。结果:氯化汞接触明显影响蟾蜍泌酥中蟾蜍甾烯水平,和正常组比较,低质量浓度(0.5 g·L^(-1))汞污染组有20种蟾蜍甾烯水平明显上调,其中有12个物质发生1.5倍变化。而中质量浓度(0.75 g·L^(-1)),高质量浓度(1.0 g·L^(-1))污染组分别有15,13种物质发生下调,且大部分为中低脂溶性蟾蜍甾烯(异沙蟾毒精、去乙酰华蟾毒精等)。结论:重金属汞污染对蟾蜍甾烯水平有潜在影响,且随着浓度的升高呈现明显降低趋势。
Objective： To investigate the effects of HgCl_2 pollution on the physiological status and bufadienolides level of toad by using liquid chromatography-mass spectrometry（LC-MS/MS）, and provide references for quality evaluation of Chinese medicine venenum bufonis. Method： The different concentrations of HgCl_2solution（0. 5,0. 75 and 1. 0 g·L-1） were prepared to treat the toad with semi-static water method,and then their physiological status was observed. Venenum bufonis was collected after 30 days. Furthermore, ultra performance liquid chromatography-mass spectrometry was used to determine the level of bufadienolides in toad venom and Hem I heat map software was used for data analysis. Result： The results showed that HgCl_2 solution could significantly influence the bufadienolides level. As compared with normal group,20 kinds of bufadienolides were up-regulated in low concentration（0. 5 g·L-1） Hg2＋exposure group, and there were 12 kinds of bufadienolides with 1. 5 fold change. However,15 kinds of bufadienolides in 0. 75 g·L-1Hg2＋exposure group and13 kinds in 1. 0 g ·L-1Hg2＋exposure group were down-regulated,and most of them were medium and low fatsoluble toad steroids（bufarenogin,dehydrated cinobufagin,etc.）. Conclusion： These illustrated that Hg2＋exposure could affect the levels of bufadienolides in toad venom,showing an obvious decrease tendency with the increase of HgCl_2 solution concentration.
作者
牛会霞
周婧
马宏跃
钱大玮
王佳佳
NIU Hui-xia ZHOU Jing MA Hong-yue QIAN Da-wei WANG Jia-jia(Jiangsu Key Laboratory for High Technology Research of Traditional Chinese Medicine Formulae and Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2017年第10期39-43,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81102762
3901894
81274199)
江苏省方剂高技术研究重点实验室/江苏省中药资源产业化过程协同创新中心资助项目(FJGJS-2015-15
ZDXM-1-14)
江苏省科技厅产学研项目(BY2015008-01)
江苏省六大人才高峰C类项目(YY-015)
江苏高校优势学科建设工程资助项目(PAPD)
关键词
汞离子
液质联用
蟾蜍甾烯
蟾蜍
泌酥
Hg2＋
LC-MS/MS
bufadienolides
Bufo bufo gargarizans
secrete Bufonis Venenum
