摘要
水稻的垩白性状是当前限制中国稻米品质提升的最主要因素。研究垩白形成机理及遗传特性,将有利于提高育种中垩白性状的改良效率。本课题组先前构建了广陆矮4号/佳辐占重组自交系(GJ RIL)及遗传图谱。本研究连续2年在上杭县和龙海市两地共种植6季GJ RILs,据各季垩白性状表型数据进行遗传分析,结合遗传图谱进行QTL定位、上位性分析和环境效应分析。遗传分析发现垩白粒率和垩白度呈偏态分布,推测垩白性状受主效基因与微效基因共同影响。QTL定位中,垩白粒率获得3个QTLs,qPGWC2、qPGWC4和qPGWC5,遗传贡献率分别为2.84%、3.74%和14.09%;垩白度获得3个QTLs,qDEC1、qDEC4和qDEC5,遗传贡献率分别为2.96%、4.88%和7.79%。上位性分析中,垩白粒率和垩白度分别获得7对和5对上位性QTLs,贡献率为0.23%~3.55%。RM307~RM518区间内同时检测到垩白粒率和垩白度的QTLs,并参与了垩白粒率和垩白度的上位性互作。RM598~RM5140区间内也同时检测到垩白粒率和垩白度的QTLs,也参与了垩白度的上位性互作。环境效应分析发现,垩白度的3个QTLs及eqDEC10和eqDEC9这对上位性QTLs均与2010年早季龙海种植环境发生显著或极显著的互作效应。
Rice （Oryza sativa L.） chalkiness is the most important limiting factor for currently improving rice quality in China. Studying rice chalky formation mechanism and hereditary character will be helpful for increasing the efficiency of the improvement of chalky quality in breeding. A Guanglu＇ai No.4/Jiafuzhan recombinant inbred line （GJ RIL） was developed and its genetic map was constructed previously by our research group. In this study, the genetic analysis of rice chalkiness was carried out basing on the chalky phenotyping data ofGJ RIL from a total of 6 growth seasons of two different locations, Shanghang county and Longhai city, in two consecutive years. Then the QTL mapping, epistasis and environmental effects of chalkiness were studied by using these phenotying data and the genetic map. The genetic analysis indicated that percentage of grains with chalkiness （PGWC） and degree of endosperm chalkiness （DEC） showed skewness distribution, suggesting that chalkiness trait was affected by both major and minor genes together. In QTL mapping, three PGWC QTLs, qPGWC2, qPGWC4 and qPGWC5, were detected, which explained 2.84%, 3.74% and 14.09% of the genetic variation, respectively. Three DEC QTLs,qDEC1, qDEC4 and qDEC5, were mapped, which explained 2.96%, 4.88% and 7.79% of the genetic variation, respectively. In QTL epistasis analysis, 7 and 5 pairs of epistasis QTLs for PGWC and DEC were identified respectively, and their contribution rates ranged from 0.23% to 3.55%. The RM307-RM518 interval harbored the PGWC and DEC QTLs, which were involved in epistatic interaction of PGWC and DEC respectively. The RM598-RM5140 interval also harbored the PGWC and DEC QTLs, which also participated in epistatic interaction of DEC. Environmental effect analysis showed the three DEC QTLs （qDEC1, qDEC4 and qDEC5） and a pair of epistatic QTLs between ^eqDECIO and ^eqDEC9 all exhibited significant or very closely significant interaction effects under the environmental conditions in early season at Longhai city of Fujian province in 2010.
出处
《分子植物育种》
CAS
CSCD
北大核心
2017年第4期1385-1394,共10页
Molecular Plant Breeding
基金
福建省中青年教师教育科研项目(JAT160014)
厦门大学中央高校科研基本业务费(2010121088
2013121-040)共同资助
关键词
水稻
垩白
数量性状遗传座位
上位性分析
Rice （Oryza sativa L.）, Chalkiness, Quantitative trait loci, Epistasis analysis
