摘要
以人参须根粉为原料,盐酸为催化剂,水饱和正丁醇溶液为提取溶剂,研究常压下加热回流直接提取人参须根粉中的人参皂苷Rg5,通过单因素实验和正交实验优化人参皂苷Rg5的提取工艺,同时进行精密度实验、稳定性实验、重复性实验和加样回收率实验,采用高效液相色谱法对人参须根粉提取物中的人参皂苷Rg5进行定量分析。结果表明,当固液比为1∶20 g/mL,酸浓度为0.06mol/L,于70℃提取5 h,人参皂苷Rg5的得率为2.48%。人参皂苷Rg5在48 h内表现出良好的稳定性,加样回收率在99.32%~103.70%之间,RSD为1.33%。该方法较大地提高了人参皂苷Rg5得率、缩短了提取时间,操作简单、重现性好。
Taking ginseng fibrous root powder （GFRP ） as raw material,hydrochloric acid as a catalyst and water - saturated n - butanol as the extraction solvent, respectively, the method of preparation of gin-senoside Rg5 from GFRP at atmospheric pressure by heating reflux was studied. The extraction process of ginsenoside Rg5 was optimized through one - factor experiment and orthogonal experiment,the precision test, stability test, repeatability test and sample recovery test were also carried out. High Performance Liquid Chromatography was applied to determine the content of ginsenoside Rg5 in ginseng fibrous root powder extracts. The optimum result obtained from orthogonal test was as follows ： ratio of ginseng fibrous root powder to extraction solvent 1 ： 20 g/mL,acid concentration 0. 06 mol/ L ,extraction temperature 70℃ and extraction time 5 h . Under the optimized conditions, the yield of ginsenoside Rg5 was 2.48%. Ginsenoside Rg5 showed good stability in 48 h ,and the sample recovery rate was 99. 3 2 % - 103.70%,RSD was 1. 33%. The method has following characteristics such as high extraction yield, simple operation,good reproducibility and short extraction time.
出处
《粮油食品科技》
2017年第3期70-76,共7页
Science and Technology of Cereals,Oils and Foods
基金
吉林省科技厅公关项目(20140306003YY)
吉林化工学院项目(吉化院合字[2015]第036号)
关键词
人参皂苷Rg5
人参须根粉
盐酸
提取
正交实验
ginsenoside Rg5
ginseng fibrous root powder
hydrochloric acid
extract
orthogonal experiment
