摘要
目的建立清肺扶正丸的质量控制标准。方法对清肺扶正丸中的熟地黄、黄芪、浙贝母和南五味子四味药材用TLC法进行定性鉴别;采用高效液相色谱(HPLC)法测定该药中毛蕊异黄酮葡萄糖苷的含量。结果黄芪、熟地黄、浙贝母和南五味子等药材薄层色谱图斑点较为清晰,阴性无干扰,具有专属性。HPLC结果显示毛蕊异黄酮葡萄糖苷含量在0.116~1.16μg范围内与峰面积呈良好线性关系(r=0.999 92,n=6),平均回收率为102.73%,RSD值为1.45%(n=9)。结论此方法专属性强、精密度高、重复性好,可为建立清肺扶正丸药品的质量控制提供依据。
Objective To establish the quality control standard of Qingfeifuzheng Pills.Methods Traditional Chinese medicines in the prescription such as Astragalus membranaceus,Thunberg fritillary bulb,Radix Rehmanniae praeparata,Schisandra chinensis were identified by TLC.The content of calycosin-7-glucoside in Qingfeifuzheng Pills was determined by HPLC.Results The spots in TLC were clear and could be well separated,the corresponding blank and adjuvants showed no interference;the HPLC method showed that the linear range of calycosin-7-glucoside was 0.116-1.16μg（r=0.999 92,n=6）;the average recovery rate was102.73%,RSD=1.45%（n=9）.Conclusion The methods were good in repeatability and reliable in the detection results,and the study provides a reference for establishing the quality control method of Qingfeifuzheng Pills.
出处
《西北药学杂志》
CAS
2017年第3期294-298,共5页
Northwest Pharmaceutical Journal
