摘要
目的:探讨AMPK在脂肪细胞分化过程中的作用以及与脂滴相关表面蛋白Cidec的表达关系,为肥胖发生及其防治肥胖及肥胖相关性疾病提供重要的理论依据。方法:通过免疫组织化学、Real-time PCR和Western blot等方法分析AMPK和Cidec在脂肪细胞分化中的作用,明确二者的相关性。结果:在不同分化程度的脂肪源性肿瘤组织中,AMPK表达随着脂肪细胞分化程度的升高而表达降低,而Cidec的表达是逐渐增高的;在不同发育阶段的胎儿脂肪组织中,AMPK随着胎龄的增加表达逐渐降低(P<0.01),而Cidec的表达则呈逐渐增高的趋势(P<0.01);以上AMPKα的表达均与Cidec的表达水平呈负相关。结论:AMPK可能在脂肪细胞分化过程中扮演重要角色,研究其与Cidec的表达与作用关系可能为脂肪细胞发育及分化提供重要线索及依据。
Objective：In this study,we would emphatically study the role of AMPK in the process of adipocyte differentiation and dig out its possible relationship with Cidec during adipogenesis,in order to provide a potential role for AMPKα as a target in treating obesity or obesity-related diseases.Methods：Using IHC staining,real-time polymerase chain reaction （RT-PCR） and western blot analysis to detect AMPK and Cidec expression in different differentiation phase of human adipocytic tumors,as well as different developmental stages of human fetal adipose tissue.Results：The results showed that AMPK was gradually increased with a decrease in differentiation of the tumor cells,while Cidec was gradually decreased;and in the fetal adipose tissues,AMPK decreased （P〈0.01） and Cidec increased （P〈0.01） significantly with the birth age increased.It was implied that AMPK was closely related with adipocytes differentiation,and showed a reverse trend compared with Cidec during the differentiation course of human adipocytes.Conclusion：In this study,we demonstrated that AMPK played a key role during the differentiation of adipocytes,thus might propose important clues to discover new possible mechanism for human adipogenesis.
出处
《现代生物医学进展》
CAS
2017年第15期2833-2836,2852,共5页
Progress in Modern Biomedicine
基金
国家自然科学基金项目(31400722)
