摘要
建立连翘叶抗氧化谱效相关质量评价指标,探索连翘叶抗氧化主要活性成分。用高效液相色谱(HPLC)法研究18个不同产地连翘叶提取物的指纹图谱;分别采用1,1-二苯基-2-苦肼基(DPPH·)清除法和Folin-Ciocalteu法测定了连翘叶的抗氧化活性和总酚含量。通过抗氧化活性和总酚含量的双变量相关分析,结果显示连翘叶抗氧化活性与其总酚含量具有较高的相关性;通过主成分、双变量相关、多元线性回归进一步分析了连翘叶抗氧化的谱效关系,结果显示连翘叶中主要有3个化学成分与其抗氧化活性之间存在着明显的关联性。本研究结果为连翘叶抗氧化活性的评价提供了依据。
A spectrum-effect correlation pattern was developed to evaluate the antioxidant activity and the main active components of Forsythia suspense leaves in this study. The fingerprint ehromatogram of the 18 Forsythia suspense leaves extracts collected from different places was established by HPLC. In addition the antioxidant activity and the content of total phenol of these 18 extracts were studied using the 1,1-diphenyl-2-picrylhydrazyl free radical（ DPPH · ）-scavenging method and Folin-Ciocalteuas method, respectively. The results showed that the antioxidant activity was correlated to the content of total phenol by the analysis of bivariate correlation. Furthermore, three components played a role in antioxidant activity of F. suspense leaves through the analysis of principal component, bivariate correlation and multiple linear regres- sion. The study provided reference for the evaluation of antioxidant activity of F. suspense leaves.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2017年第4期629-634,共6页
Natural Product Research and Development
基金
山西省国际合作项目(2014081049-1)
山西省科学技术发展计划(2014ZDO0402)
关键词
连翘叶
指纹图谱
抗氧化
谱效相关
Forsythia suspense leaves
fingerprint
antioxidant activity
spectrum-effect correlation pattern
