摘要
通过酰化反应将叶酸(FA)、两亲性亚油酰化壳寡糖(LCOS)连接到羧基取代的四苯基卟啉(TPP)化合物5-(4-羧基苯基)-10,15,20-三苯基卟啉(CPTPP)结构上,得到了潜在光敏功能化载药系统CPTPP-FA-LCOS。利用FTIR、UV-Vis、TEM、FL对CPTPP-FA-LCOS的结构进行了表征,并运用表面张力法测定了其临界胶束质量浓度(CMC)。结果表明:CPTPP-FA-LCOS的CMC=2.5×10^(-3)g/L,其可形成稳定的球形纳米胶束,粒径在70~120 nm。四唑盐(MTT)比色法的初步实验结果表明:在光照条件下,0.4 g/L的CPTPP-FA-LCOS即可使He La细胞存活率降至50%以下,表明CPTPP-FA-LCOS具有明显的光毒性。
A photosensitive drug delivery system CFFPP-FA-LCOS was obtained by acylation reaction of folic acid （FA）, amphiphilic N-linoleyl chitosan oligosaccharide （LCOS） with carboxyl substituted meso-tetraphenylporphyrin （TPP） of 5- （4-carboxyl） phenyl-10,15,20-triphenyl porphyrin （ CPTPP ）. The chemical structure of CPTPP-FA-LCOS was characterized by FTIR, UV-Vis,TEM, and fluorescence spectrum. The critical micefle concentration （CMC） of CFI＇PP-FA-LCOS was also determined using surface tension method. The results showed that CMC value of CPTPP-FA-LCOS was 2.5× 10-3 g/L. CPTPP-FA-LCOS could form stable spherical nano-micelles with a particle size between 70 and 120 nm. The results of preliminary MTI＂ （methyl thiazolyl tetrazolim ） suggested that 0. 4 g/L CPTPP-FA-LCOS could make the HeLa cell viability reduce to less than 50% under the light condition, indicating that CPTPP-FA-LCOS had obvious phototoxicity.
出处
《精细化工》
EI
CAS
CSCD
北大核心
2017年第5期494-498,共5页
Fine Chemicals
基金
国家自然科学基金(21601068/21471069/21302071)
江苏省高等自然科学研究面上项目(14KJB150006)
江苏省博士后科研资助计划项目(1401050C)
江苏大学高级人才科研启动基金(14JDG054)~~
关键词
光动力疗法
光敏剂
亚油酰化壳寡糖
叶酸
功能材料
photodynamic therapy
photosensitizer
N-linoleyl chitosan oligosaccharide
folic acid
functional materials
