摘要
目的评价氢对大鼠脑缺血再灌注时脑组织caspase-3活化的影响。方法健康成年雄性sD大鼠36只，体重220～250g，采用随机数字表法分为3组（n=12）：假手术组（s组）、缺血再灌注组（I／R组）和氢组（H组）。采用大脑中动脉闭塞再灌注法制备脑缺血再灌注损伤模型。H组于模型制备前3d及再灌注即刻腹腔注射富氢液5ml／kg（0．6mmol／L）。于再灌注24h时处死，取海马组织，采用TUNEL法检测神经细胞凋亡情况，计数凋亡神经细胞，采用Westernblot法测定活化caspase-3的表达水平。取缺血区组织HE染色后，观察病理学结果。结果与s组比较，I／R组和H组海马活化caspase-3表达上调，凋亡神经细胞计数升高（P〈0．05）；与I／R组比较，H组海马活化caspase-3表达下调，凋亡神经细胞计数降低（P〈0．05），脑组织病理学损伤减轻。结论氢抑制大鼠脑缺血再灌注时神经细胞凋亡的机制可能与抑制脑组织caspase-3活化有关。
Objective To evaluate the effect of hydrogen on the activation of caspase-3 in brain tissues during cerebral ischemia-reperfusion （I/R） in rats. Methods Thirty-six healthy adult male Sprague- Dawley rats, weighing 220-250 g, were divided into 3 groups （n= 12 each） using a random number table： sham operation （ group S） , I/R group and hydrogen group （ group H）. Cerebral ischemia was induced by occlusion of the middle cerebral artery followed by reperfusion in I/R and H groups. In group H, hydrogen- rich saline 5 ml/kg （0. 6 mmol/L） was injected intraperitoneally at 3 days before establishment of the model and immediately after the onset of reperfusion. At 24 h of reperfusion, the rats were sacrificed, and hippocampal tissues were obtained for determination of neuroapoptosis （ by TUNEL） , apoptotic neuron count and expression of activated caspase-3 （ by Western blot）. The brain tissues in the ischemic area were ob- tained and stained with haematoxylin and eosin for examination of the pathological changes. Results Com- pared with group S, the expression of activated caspase-3 was significantly up-regulated, and the apoptotic neuron count was increased in I/R and H groups （P〈0. 05）. Compared with group I/R, the expression of activated caspase-3 was significantly down-regulated, the apoptotic neuron count was decreased （P〈0. 05） , and the pathological changes of brain tissues were significantly reduced in group H. Conclusion The mech- anism by which hydrogen inhibits neuroapoptosis during cerebral I/R is probably related to inhibited activation of caspase-3 in brain tissues of rats.
出处
《中华麻醉学杂志》
CSCD
北大核心
2017年第3期305-307,共3页
Chinese Journal of Anesthesiology
