摘要
【目的】利用响应面法对微波辅助提取七叶一枝花总黄酮的工艺条件进行优化,以期提高七叶一枝花总黄酮提取量,为实际生产提供技术支撑。【方法】在单因素试验的基础上,选取乙醇体积分数、液料比和微波功率3个主要影响因素进行Box-Behnken中心组合试验设计,筛选七叶一枝花总黄酮提取最佳工艺参数。【结果】建立了七叶一枝花总黄酮提取量(Y)与乙醇体积分数(A)、液料比(B)和微波功率(C)的回归模型:Y=4.90+0.30A+0.51B+0.48C-0.19AB+0.44AC+0.24BC-0.85A^2-0.83B^2-0.55C^2(R^2=0.9734),拟合程度较高。3个影响因素及乙醇体积分数与微波功率的交互作用对七叶一枝花总黄酮提取量有极显著影响(P<0.01);微波辅助提取七叶一枝花总黄酮的最佳工艺条件:乙醇体积分数50%、液料比17∶1、微波功率528 W,在此条件下七叶一枝花总黄酮提取量为5.28 mg/g,与预测值(5.18 mg/g)的相对误差为1.93%。【结论】以响应面法优化微波辅助提取七叶一枝花总黄酮工艺参数具有一定的可行性,可用于实际生产。
[Objective]In the present study, response surface methodology was applied to optimize the process condi- tions for the microwave-assisted extraction of total flavonoids from Paris Polyphylla, in order to improve the extraction yield of total flavonoids and provide technique support for actual production. [ Method ] Based on single factor experiments, three major parameters including alcohol volume fraction, microwave power and liquid-solid ratio were optimized using Box- Behnken certral composite design, in a bid to select the optimal process parameters for total flavonoids extraction from P. Polyphylla. [Result]The regression model based on extraction of total flavonoids from P. Polyphylla（Y）, ethanol volume fraction（A）, liquid-solid ratio（B） and microwave power（C） was as follows：Y=4.90＋0.30A＋0.51B＋0.48C-0.19AB＋0.44AC＋ 0.24BC-O.85A2-0.83B2-0.55C2（RZ=0.9734）. The fitting degree was high. The three parameters and interaction between ethanol volume fraction and microwave power had extremely significant influence on total flavonoids extraction yield （P〈 0.01）. The optimal extraction conditions were as follows： ethanol volume flaction 50%, liquid-solid ratio 17：1 and microwave power 528 W. Under such conditions, the extraction yield was 5.28 mg/g. The relative error was 1.93% compared to the predicted yield （5.18 mg/g）. [Conclusion]Parameters of microwave-assisted total flavonoids extraction from P. Polyphylla optimized by response surface methodology are feasible, which can be applied to actual production.
出处
《南方农业学报》
CAS
CSCD
北大核心
2017年第4期698-703,共6页
Journal of Southern Agriculture
基金
河南省科技计划项目(162102310587)
关键词
七叶一枝花
总黄酮
微波辅助提取
响应面法
工艺优化
Paris Polyphylla
total flavonoids
microwave-assisted extraction
response surface methodology
pro- cess optimization
