摘要
2015年11月中旬采集常州滆湖底泥及水样,在滆湖水体及底泥现状分析的基础上,采用高通量测序技术对底泥细菌的16S rRNA基因进行序列测定,对底泥细菌群落结构及多样性进行研究.结果显示:滆湖水质整体呈富营养化状态,殷村港、湖北区为轻度富营养化,繁保区、塘门沟为中度富营养化.底泥细菌测得总OTUs(Operational Taxonomic Units)数为6157,多样性高.滆湖底泥细菌隶属26门38纲141属,其中以Proteobacteria、Chloroflexi、Deltaproteobacteria、Betaproteobacteria和Deltaproteobacteriawei为主,并含有少量丰度的Planctomycetacia、Alphaproteobacteria、Thiobacillus、Polynucleobacter和Geobacter等.研究发现,Firmicutes和Alphaproteobacteria丰度与富营养化指数呈显著正相关,而Betaproteobacteria、Actinobacteria和Bacteroidetes丰度与富营养化指数呈显著负相关,即富营养化程度越高,Firmicutes和Alphaproteobacteria丰度也越大,Betaproteobacteria、Bacteroidete和Actinobacterias丰度则越小.
The sediment and water samples are collected at Gehu Lake of Changzhou city in the middle of November in 2015.The sequence of 16 S rRNA of the sediment bacteria is determined by Sing Pyrosequencing.The structure and diversity of sediment microbial communities are studied on the basis of analysis of the water quality and sediment contents.The results show that Gehu Lake is eutrophic as a whole,Yincungang site and Hubeiqu area are lightly eutrophicated,and Fanbaoqu and Tangmengou zones are moderate.The total number of OTUs（Operational Taxonomic Units） is 6157 according to testing sediment bacteria,which shows a remarkable diversity.The sediment microbes in Gehu Lake are subordinate to 26 phylum,38 classes and 141 genus,in which most of the bacterial are Proteobacteria,Chloroflexi,Deltaproteobacteria,Betaproteobacteria and Deltaproteobacteriawei and there is a tiny amount of Planctomycetacia,Alphaproteobacteria,Thiobacillus,Polynucleobacte,Geobacter and so on.It indicates that the abundance of Firmicute and Alphaproteobacteria increases with the increased eutrophication degree,but the abundance of Betaproteobacteria,Actinobacteria and Bacteroidetes decreased on the contrary.In other words,the abundance of Firmicutes and Alphaproteobacteria is greater,and that of Betaproteobacteria,Bacteroidete and Actinobacterias is smaller as eutrophication is more serious.
出处
《环境科学学报》
CAS
CSCD
北大核心
2017年第5期1649-1656,共8页
Acta Scientiae Circumstantiae
基金
国家"十二五"水体污染控制与治理科技重大专项(No.2012ZX07101-007)
江苏省环保科研重点研究课题(No.2013038)~~
关键词
底泥
高通量
细菌
群落组成
滆湖
sediment
pyrosequencing
bacterial
composition communities
Gehu Lake
