摘要
运用正交设计法,以总黄酮的得率及其抗氧化能力为评价指标,考察提取次数、溶剂浓度、提取时间、料液比4个因素对红芪总黄酮提取工艺的影响;紫外吸收法测定红芪总黄酮的含量,DPPH法测定总黄酮的抗氧化能力。结果表明,红芪总黄酮的最佳提取工艺为:药材加8倍量的65%乙醇,回流提取3次,每次40 min。在此条件下红芪总黄酮的提取率25.04%,抗氧化能力最佳,DPPH自由基清除率53.55%。
Using orthogonal design method, with total flavonoids yield and the antioxidant capacity of strength as the evaluation index. The effects of extraction times,solvent concentration,extraction time,sol-id to liquid than the four factors of total flavonoids of Radix Hedysari extraction effect; ultraviolet absorp-tion method for the determination of the content of total flavonoids of Radix Hedysari, DPPH method deter-mination of total flavonoids antioxidant capacity. Results showed that the optimum extraction process of to-tal flavonoids of Radix Hedysari-.herhs plus 8 times the amount of 65% ethanol, extracting 3 times, each time 40 min. Under these conditions, the extraction yield of total flavonoids of Radix Hedysari was 25.04% ,and the antioxidant capacity is the best,and the DPPH free radical clearance was 53.55%.
出处
《应用化工》
CAS
CSCD
北大核心
2017年第5期928-930,共3页
Applied Chemical Industry
基金
2013甘肃省科技厅项目(1308RJZA110)
甘肃省自然科学基金(148RJZA061)
2015年度甘肃中医药大学中青年科研基金项目(ZQ2015-4)
2016年甘肃省中药质量与标准研究重点实验室培育基地开放基金项目计划(ZYZL16-013)
