摘要
目的研究添加与不加甲苯对24 h尿微量总蛋白(MPr)和尿微量白蛋白(UMA)定量测定的影响。方法收集各个浓度段门诊及住院病人的新鲜随机尿共82份,检测即刻与在低、中、高温度条件下,加和不加甲苯保存24 h后尿液MPr和UMA的浓度,通过配对t检验方法检验差异有无统计学意义。结果即刻尿MPr的值与低中温条件下、有无甲苯的24 h尿MPr的检测值比较,差异均无统计学意义(P>0.05),但与在高温条件有无甲苯检测值比较,差异均有统计学意义(P<0.05);即刻尿UMA的值与低中温条件下、有无甲苯的24 h尿UMA检测值比较,差异均无统计学意义(P>0.05),与高温条件下有无甲苯检测值相比,差异均有统计学意义(P<0.05);同一温度保存24 h,得到尿MPr、尿UMA的检测值,有无甲苯的检测结果比较,差异均无统计学意义(P>0.05)。结论实验证明甲苯在24h尿MPr与尿UMA的定量检测中无使用的必要性。
Objective To investigate the effect of toluene in the quantitative detection of 24 hour （24 h） urine micro protein （MPr） and urine micro albumin （UMA）. Methods Eighty-two random urine of the patients with different concentrations were collected. The group without toluene and the other group with toluene were stored at the low, medium and high tcmpreture for 24 h respectively. Then MPr and UMA were measured in 0 h and 24 h. Paired t-test was used to test whether the difference was statistically significant. Results When stored at the low and medium temperature, the results of immediate detection of MPr and UMA were compared with those of the addition of toluene or without for 24 h. The difference was not statistically significant （ P 〉 0. 05 ）. However, there was significant difference in MPr and UMA value of urine at high temperature under the same comparison （ P 〈 0. 05 ）. Stored at the same temperature for 24 hours, the results of MPr and UMA were compared between the adding groups and the non-adding groups. The results showed that there was no significant difference between them. Conclusion The specimens for the quantitative detection of the 24 h MPr and UMA are not need to be added with toluene, and toluene in the urine is not preservatively effective.
出处
《广州医药》
2017年第3期64-67,共4页
Guangzhou Medical Journal
