摘要
目的:制备大鼠的溃疡性结肠炎(ulcerative colitis,UC)模型,并制备大黄结肠靶向微丸,考察微丸对大鼠UC的干预作用。方法:采用海藻酸钠、果胶和壳聚糖通过滴制法制备丸芯,并包以肠溶衣制成大黄结肠靶向微丸,测定微丸中游离蒽醌含量,计算微丸的载药量。Wistar大鼠采用噁唑酮建立UC模型,造模前随机分为预保护组(3个剂量组:200、140、80 mg·kg^(-1)),正常组和模型组,每组各6只,预保护组在造模前3 d开始灌胃微丸,连续给药8 d,d 9处死,剖腹取结肠,称量结肠湿质量,观察结肠损伤情况,计算结肠湿质量指数并对大鼠结肠病变进行评分。结果:大黄结肠靶向微丸预保护组140 mg·kg^(-1)剂量组的结肠湿质量指数(7.86、6.20、7.29、7.59、6.11、4.65)(P<0.05)和结肠病变肉眼评分(P<0.01)与模型组相比差异有显著性,可明显改善UC大鼠结肠组织的病理损伤。结论:大黄结肠靶向微丸对UC大鼠具有一定预保护作用。
Objective：To prepare the rhubarb colon-specific pellets,and study on the protection of the pellets in ulcerative colitis （UC） rats. Methods：Sodium alginate,pectin and chitosan were used to prepare the pill-core by dropping preparation method. The oxazolone （OXZ） was used to set up UC model. Wistar rats were randomly divided into protection groups （3 dosage groups：200,140,80 mg.kg-1）,normal group and model group before the modeling, each group include 6 rats, male and female half. Protection groups were given pellets by gavage 3 days before the modeling for 8 d with rhubarb colon-specific pellets. The rats were executed at d 9 and the colon tissues were harvested through laparotomy. The wet quality of colon tissues was weighed, and the colon injury was observed. The colon wet quality index was calculated and the colon lesions of rats were graded. Results ：The colon wet quality index （7.86,6.20,7.29, 7.59,6.11,4.65） （P〈0.05） and colon lesions to the naked eye score （P〈0.01） of the 140 mg. kg-1 group have significant difference with model group. The colon tissue pathological damage of the UC rats can be obviously improved with the 140 mg.kg-1 of rhubarb colon-specific pellets.Conclusion：Rhubarb colon-specific pellets have certain protective effect on UC rats.
出处
《神经药理学报》
2016年第2期14-19,共6页
Acta Neuropharmacologica
基金
国家自然科学基金项目(No.81274005)
