摘要
目的:观察丁苯酞对颅脑损伤大鼠脑组织含水量、血清神经生化标志物S100B和神经元特异性烯醇化酶(NSE)蛋白浓度的影响。方法:110只健康雄性SD大鼠,随机分为假手术组(n=20)、模型组(n=30)、吡拉西坦治疗组(阳性对照组,n=30)和丁苯酞治疗组(丁苯酞组,n=30)。后三组大鼠参考Feeney法复制颅脑损伤模型。成模后24h,阳性对照组给予吡拉西坦溶液(3.6g/kg/天)灌胃,丁苯酞组给予丁苯酞溶液(160mg/kg/天)灌胃,其余两组平行灌胃等量纯化水,均每天一次,连续10天。比较各组大鼠治疗期间死亡率;于药物干预第3天和第10天分批心脏取血及处死大鼠取脑组织,检测各组大鼠血清S100B和NSE蛋白含量和脑组织含水量。结果:治疗过程中丁苯酞组大鼠死亡率较模型组降低(P<0.01),与阳性对照组无明显差异(P>0.05)。治疗后第3、第10天,各组血清S100B、NSE蛋白浓度和脑组织含水量差异均有统计学意义(P<0.01)。模型组较假手术组血清S100B、NSE蛋白浓度和脑组织含水量明显升高(P<0.05,P<0.01);阳性对照组及丁苯酞组较模型组降低(P<0.05,P<0.01);阳性对照组与丁苯酞组差异无统计学意义(P>0.05)。阳性对照组和丁苯酞组血清S100B、NSE蛋白浓度和脑组织含水量在治疗后第10天较第3天下降更明显(P<0.01)。结论:丁苯酞能改善颅脑损伤后大鼠脑组织水肿,降低血清S100B和NSE蛋白含量,疗效与吡拉西坦相当,具有脑保护作用。
Objective： To explore the effect of dl-3n-butyphthalide（NBP）on the content of brain water,serum S100B and neuron specific enolase （NSE） in traumatic brain injuried rats. Method： 110 healthy male SD rats were randomly divided into the sham group （n= 20）, model group（n=30）, piracetam treatment group（the positive control group,n=30）, and NBP treatment group（NBP group, n=30）. The last three groups were made into models. The brain injury model was induced by free-falling method. Then drug was used after the traumatic brain injuriy. The positive control group was intragastrie administration with pyrazole raschig solution（3. 6g/kg/d）. The NBP group was intragastric administration with Butylphthalide solution（160mg/kg/d）. Blood samples were collected from heart on 3rd and 10th day after the drug intervention. The expression of serum S100B and NSE levels were detected by ELISA. The content of brain water of some killed rats from each group was compared. Results： Death rate in model group was higher than positive group and NBP group （P〈0.01）. The 3rd and 10th day after traumatic brain injury, compared with the sham group, the content of brain water,serum S100B and NSE in traumatic brain injuried rats were all increased（P〈 0. 01）. Compared with the model group, the content of brain water, serum S100B and NSE in NBP group and the positive group were all decreased（P〈0.01）. There was no statistically significant in positive control group and NBP group （P〉0.05）. Compared with the 3rd day, above detection targets on the 10th day were decreased in positive control group and NBP group （P〈0. 01）. Conclusion： NBP can improve tissue edema rats after traumatic brain injury, down-regulate S100B and NSE level in blood. It＇s efficacy is equal to piratetam. NBP has the neuroprotective effect against traumatic brain injury.
作者
喻小平
陈谦学
YU Xiao-ping CHEN Qian-xue(Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan 430060, Chin)
出处
《微循环学杂志》
2017年第2期17-20,共4页
Chinese Journal of Microcirculation
