摘要
在单因素试验基础上应用正交试验方法对芒果核多酚提取条件进行优化并初步评价其体外抗氧化活性。试验确定乙醇为最佳提取溶剂;各因素对多酚物质提取量的影响依次为料液比>乙醇提取浓度=提取时间>提取温度;用乙醇溶液提取芒果果核中多酚物质的最佳工艺条件为乙醇浓度70%,料液比1∶25(g/m L),提取时间120 min,提取温度60℃,芒果核多酚物质提取含量可达4.36 mg/g。抗氧化活性试验结果表明芒果核多酚物质对羟基自由基、超氧阴离子自由基及DPPH自由基的清除率分别为90.9%、83.3%、90%。优化的芒果核多酚提取工艺合理、可行,芒果核多酚物质具有较强的抗氧化性。
Based on the single factor experiments, orthogonal experimental design was employed to optimize the extraction conditions of polyphenol from mango kernel seeds and its antioxidative activity was evaluated. The results revealed the ethanol to be the best extraction solvent extraction and determined the factors affect the amount of time for the extraction as followed ： ratio of raw material to water 〉 concentration of ethanol = time〉 temperature. The optimum extraction condition were ： concentration of ethanol 70 %, ratio of water to raw mate- rial 1 ： 25 （g/mL） , extraction time 120 min, extraction temperature 60 ℃. Under these conditions, the extraction yield of polyphenol was 4.36 mg/g. The results of antioxidation experiments showed that the highest scav- enging capacity of polyphenol against · OH, O2-· and DPPH free radical was 90.9 %, 83.3 % and 90 %, respectively. The optimization of polyphenol extraction process technology was reasonable and feasible, polyphenol from mango kernel seeds had stronger antioxidant activity.
出处
《食品研究与开发》
CAS
北大核心
2017年第5期47-51,共5页
Food Research and Development
基金
国家自然科学基金(21365011)
广西自然科学基金(2013GXNSFAA019046)
贺州学院博士启动基金(HZUBS201515)
关键词
芒果核
多酚物质
抗氧化活性
mango kernel seeds
polyphenol
ant/oxidative activity
