摘要
目的研究丝素蛋白/聚己内酯(silk fibroin,SF/polycaprolactone,PCL)制备的三维支架的生物相容性及降解性能。方法利用高压静电纺丝技术将SF/PCL溶液制备成三维支架式的纤维膜性结构。通过添加下颌骨髁状突软骨细胞(mandibular condylar chondrocytes,MCCs)培养液与无细胞培养液,将SF/PCL三维支架膜分为实验组、对照组。通过观测SF/PCL三维支架膜上软骨细胞的生长情况探索其生物相容性;通过体外降解实验观测1、2、3、4、5、6、7dSF/PCL三维支架的纤维膜剩余重量,分析其降解性能。结果扫描电镜(scanning electron microscopy,SEM)下观察显示,12h时髁突软骨细胞在纤维膜上正常贴壁生长,1d后逐渐分泌大量胞外基质。体外降解实验结果显示,无细胞添加组的剩余重量随降解时间的变化小于细胞添加组(P<0.05)。结论 SF/PCL三维支架具有良好的生物相容性和可降解性,该研究为进一步研究生物支架修复软骨组织损伤或缺损提供了实验依据。
Objective To examine the biocompatibility and degradation of three-dimensional scaffolds prepared by silk fibroin（SF）/polycaprolactone（PCL）. Methods High-voltage electrospinning was used to prepare SF/PCL solution into a three- dimensional membranous structure. The SF/PCL three-dimensional scaffolds were divided into the experimental group and con- troI group by adding the culture medium with mandibuIar condylar chondrocytes（MCCs）and cell-free culture medium, respec- tively. The growth of MCCs was observed on the membrane to determine the biocompatibility of SF/PCL three-dimensional scaffolds. In vitro degradation tests were utilized to measure the residual weight of the fibrous membrane at 1,2,3,4,5,6 and 7 d to analyze the degradation of the SF/PCL three-dimensional scaffolds. Results Scanning electron microscopy（SEM）showed that adhering to the SF/PCL scaffold, MCCs grew normally at 12 h, and they began to secret a large amount of extracellular ma- trix 1 d later. Degradation experiments indicated that the residual weight chanKed less in non-cell group than in cell-added group （P〈0.05）. Conclusion The SF/PCL three-dimensional scaffold has excellent bi0compatibility and biodegradability,which pro- vides experimental basis for further study on repairing of cartilage injury or defect with the biological scaffold.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2017年第2期165-169,共5页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
基金
国家自然科学基金资助项目(No.81360163)
浙江省医药卫生科技项目(No.2016KYB338)
关键词
组织工程支架
生物相容性
体外降解
静电纺丝
tissue engineering scaffolds
biological compatibility
in vitro degradation
electrospinning
