摘要
目的探讨血清TK1和PSA检测在诊断和鉴别诊断良性前列腺疾病(前列腺增生)与前列腺癌(PCa)中的作用。方法采用酶免疫点印迹化学发光法检测:方便选取2013年1月—2015年12来内蒙古科技大学包头医学院第一附属医院就诊并确诊的100例前列腺癌患者、85例前列腺增生患者、80例正常对照者血清TK1水平,同时采用罗氏电化学发光分析仪检测PSA(TPSA/FPSA)含量。比较前列腺癌患者与前列腺良性疾病及对照组的TK1及PSA水平的差异,以及前列腺癌组患者血清TK1和PSA水平与术后复发及手术前后关系。结果前列腺癌组PSA(56.38±14.25)和TK(8.54±3.65)1水平显著高于前列腺良性肿瘤[PSA:(4.78±2.54);TK:(1.51±0.48)]和健康人群组[PSA:(1.35±1.27);TK:(0.94±0.51)],(P<0.01),且前列腺良性肿瘤组[PSA:(4.78±2.54);TK:(1.51±0.48)]与健康人群组差异有统计学意义[PSA:(1.35±1.27);TK:(0.94±0.51)],(P<0.05)。PSA和TK1联检前列腺癌其灵敏度和特异性都有所提高,(FPSA/t PSA的灵敏度为67.4%,特异度为73.5%。血清TK1的灵敏度为71.5%,特异性为78.24%,二者联合检测PCa的灵敏度位为97.54,特异度为91.38。且血清TK1水平在手术前后差异有统计学意义[手术前:tk1:(8.54±3.65);t PSA:(56.38±14.25);f PSA:(5.21±1.33);f PSA/t PSA:(0.09±0.04),手术后:tk1:(4.11±4.06);t PSA:(49.68±13.02);f PSA:(5.01±1.55);f PSA/t PSA:(0.11±0.05)。术后复发的前列腺癌患者中,TK1水平明显升高[无复发(3.06±2.16);有复发(9.65±4.89),P<0.05]。结论血清TK1可为前列腺癌预后判断及病情监测提供重要价值,与PSA联合检测能够提高前列腺癌诊断的检出率。
Objective To s tudy th e effect of serum TK1 and PSA te s t in diagnosis an d d ifferent ia l d iagnosis of p a ra s ta ta l and PCa. Methods C onvenient s ele ct ion th e serum TK1 leve l of 100 c a se s of p a t ien ts from F is t Affiliated Hosp ita l of Baotou Medical Col lege,Inner Mongolia Univeristy of Science and Technology with PCa, 85 cases of pat ients with parastatal and 80 cases of normal persons treated in our hospi tal from January 2013 to December 2015 were tes ted by the chemistry luminescence immunoassay method, at the same time, PSA （TPSA/FPSA）content was tes ted by the Roch electrochemical luminescence analyzer , and the differences in the TK1 and PSA levels of patients in the three groups were compared, and the correlation between the serum TK1 and PSA levels of patients with PCa and pos toperative recur rence was compared.Results The PSA an d TK1 levels in th e PCa group was obviously h ig h e r th an th a t in th e b en ig n n eo plasm of p ro sta te group and heal thypeople group [ （56.38±14.25） ,（8.54±3.65） vs （4.78±2.54） ,（1.51±0.48） ,（1.35±1.27） ,（0.94±0.51） , P〈0.01] , and the differences between the benign neoplasm of prostate group and healthy people group had stat ist ical s ignif icance, and the sensit ivity and specif ici ty of PSA and TK1 in test ing PCa were improved,（ the sensit ivity and specif ici ty of FPSA/tPSA were respectively 67.4% and 73.5%, and the sensit ivity and specif ici ty of serum TK1 were respectively 71.5% and 78.24%, and the sensit ivity and specif ici ty of the combined tes t were respectively 97.54 and 91.38）, and the difference in the serum TK1 level before and af ter operat ion had stat ist ical signif icance [（before operat ion, tk1：（8.54±3.65）; tPSA：（56.38±14.25） ;fPSA： （5.21±1.33） ; fPSA/ tPSA：（0.09±0.04） ; af ter operat ion; tk1：（4.11±4.06）;tPSA：（49.68±13.02） ;fPSA：（5.01±1.55） ;fPSA/ tPSA： （0.11±0.05） ] ,and TK1 level obviously increased of PCa pat ients with pos toperative recur rence [no recur rence：（3.06±2.16） ; recur rence; （9.65±4.89）, P 〈 0 .0 5 ] . Conclusion The serum TK1 c a n provide imp o rtant v alue for th e prognosis ju d gm en t anddisease monitoring of PCa, and the combined tes t of serum TK1 and PSA can improve the test rate of PCa.
出处
《中外医疗》
2017年第3期63-66,共4页
China & Foreign Medical Treatment
