摘要
目的:观察枸杞多糖(LBP)对脂多糖(LPS)致人脐静脉血管内皮细胞(HUVECs)损伤的保护作用,并探讨其可能的作用机制.方法:体外培养人脐静脉内皮细胞,分为空白对照组、LBP组、LPS模型组、LBP+LPS实验组,采用CCK-8法检测细胞活力,Western-blot法检测诱导型一氧化氮活酶(iNOS)、Caspase-3及NF-κB p65蛋白水平,ELISA法检测上清液TNF-α、IL-1β水平,Gries法测上清一氧化氮(NO)含量,硫代巴比妥酸法检测丙二醛(MDA).结果:与对照组相比,LPS模型组HUVEC增殖活力显著下降,NF-κB p65蛋白、iNOS、NO、TNF-α、IL-1β、MDA及Caspase-3水平显著升高,LBP处理则可部分增加受损内皮细胞的增殖活性,NF-κB p65蛋白、iNOS、NO、TNF-α、IL-1β、MDA及Caspase-3水平显著下降.结论:枸杞多糖对脂多糖诱导的人脐静脉内皮细胞损伤具有保护作用,与抑制NF-k B信号通路、抑制促炎因子TNF-α和IL-1β释放,抑制NO合成、减轻氧化应激,抑制凋亡蛋白Caspase-3表达有关.
Aim: To observe the protective effect of Lycium barbarum polysaccharides(LBP) on human umbilical vein endothelial cells(HUVECs) treated with lipopolysaccharide(LPS),and to explore the possible mechanisms. Methods: Human umbilical vein endothelial cells were cultured in vitro,divided into blank control group,LBP group,LPS model group and LBP + LPS group. Cell viability was detected by CCK-8 method. Western blotting was used to detect inducible nitric oxidesynthase(iNOS),and IL-1β levels were measured by ELISA. The levels of nitric oxide(NO) in supernatant were measured by Gries method. The levels of malondialdehyde(MDA) were determined using thiobarbituric acid method. Results: Compared with the control group,the proliferative activity of HUVEC decreased significantly,and the levels of NF-κB p65 protein,iNOS,NO,TNF-α,IL-1β,MDA and Caspase-3 were significantly increased in the LPS model group. NF-κB p65 protein,iNOS,NO,TNF-α,IL-1β,MDA and Caspase-3 levels were significantly decreased in the damaged endothelial cells.Conclusion: Lycium barbarum polysaccharide has protective effects on lipopolysaccharide-induced injury of human umbilical vein endothelial cells,it inhibits NF-k B signaling pathway,reduces the release of proinflammatory cytokines TNF-α and IL-1β,inhibits NO synthesis,reduces oxidative stress,and inhibits apoptosis protein Caspase-3 expression.
出处
《暨南大学学报（自然科学与医学版）》
CAS
CSCD
北大核心
2017年第3期240-246,共7页
Journal of Jinan University(Natural Science & Medicine Edition)
基金
国家自然科学基金项目(81270568)
关键词
枸杞多糖
脂多糖
人脐静脉血管内皮细胞
NF-ΚB
lycium barbarum polysaccharides
lipopolysaccharide
human umbilical vein endothelial cells
NF-kB
