摘要
目的:探讨PTP4A1基因干扰对人舌鳞癌CAL27细胞增殖、凋亡及体外迁移的影响。方法:采用PTP4A1干扰慢病毒转染人舌鳞癌CAL27细胞,实时定量PCR和Western blotting验证PTP4A1干扰效果,分别采用MTT法、划痕试验检测细胞增殖、迁移情况,流式细胞术分析细胞凋亡,Western blotting分析caspase-3、Bax、Bcl-2蛋白表达。结果:PTP4A1干扰慢病毒转染后,干扰组中CAL27细胞PTP4A1基因mRNA和蛋白表达均明显减少。与对照组比较,PTP4A1干扰后,细胞增殖、体外迁移能力均显著减弱(P<0.05);PTP4A1干扰后CAL27细胞凋亡率相比对照组显著增大(P<0.05),PTP4A1干扰促进CAL27细胞凋亡与上调caspase-3、Bax蛋白表达,下调Bcl-2蛋白表达水平有关。结论:PTP4A1基因干扰可抑制人舌鳞癌CAL27细胞增殖活性和体外迁移能力,且促进舌鳞癌细胞凋亡。
Objective：To study the effects of PTP4A1 gene interference on cell proliferation, apoptosis and migration ability in human tongue squamous cancer ceils CAL27 in vitro. Methods： Lentivirus transfection was used to realize the PTP4A1 gene interference in human tongue squamous cancer cells CAL27. PTP4A1 gene interference was verified by realtime PCR and western blotting. The cell proliferation was analysed by MTT method, and wound scratch assay was used to detect cell migration ability, the cell apoptosis rate was detected by flow cytometry. The apoptotic proteins expression levels of caspase-3, Bax and Bcl-2 were detected by western blotting. Results：The gene expression levels of PTP4A1 mRNA and protein in human tongue squamous cancer cells CAL27 were significantly decreased after interference lentivirus transfection. Af- ter the interference of PTP4A1, the cell proliferation and migratory ability of CAL27 cells in vitro were decreased （P 〈 0.05 ）. The cell apoptosis rate significantly increased after PTP4A1 gene interference（P 〈0. 05 ） ,along with the caspase-3 and Bax protein expression levels up-regulation, and the Bcl-2 expression levels down-regulation. Conclusion： Gene interference of PTP4A1 in human tongue squamous cancer cells CAL27 inhibits the cell proliferation, migration ability in vitro, and enhances the cell apoptosis rate of CAI27 cells.
出处
《临床口腔医学杂志》
2017年第4期217-220,共4页
Journal of Clinical Stomatology
关键词
人舌鳞癌细胞
PTP4A1基因
细胞增殖
凋亡
迁移
Human tongue squamous cancer
PTP4A1 gene
Cell proliferation
Apoptosis
Migration
