摘要
为探讨氟化钠对小鼠睾丸谷胱甘肽抗氧化系统的影响,将24只3周龄ICR雄性小鼠随机分为对照组和染氟组,对照组给予蒸馏水,染氟组分别供给含25、50、100mg·L^(-1) NaF的蒸馏水,自由饮用60d。染毒结束后检测小鼠睾丸总抗氧化能力(T-AOC)、活性氧(ROS)、丙二醛(MDA)、还原型谷胱甘肽(GSH)的含量及谷胱甘肽过氧化氢酶(GPx)、谷胱甘肽硫转移酶(GST)、谷胱甘肽还原酶(GR)的活性,检测GPx1、GPx4、GST-mu5和GR基因的转录。电镜观察睾丸组织细胞内线粒体、内质网和高尔基体的结构。结果显示,氟中毒可使睾丸T-AOC减弱,ROS、MDA含量升高,GSH含量显著降低,细胞内氧化水平升高,线粒体、内质网和高尔基体出现不同程度的损伤,GPx、GST、GR的活性降低,GPx1、GPx4、GST-mu5和GR基因转录下调。小鼠氟中毒后,睾丸抗氧化能力降低,处于明显的氧化应激状态,这与谷胱甘肽抗氧化系统酶活性降低及其相关基因转录下调有关。
This study was conducted to investigate the effect of sodium fluoride on the glutathione antioxidant system of mice testis. Twenty-four 3 weeks old ICR male mice were randomly divided into control group and 3 fluoride groups. The four groups were orally administrated with 0, 25, 50, and 100 mg·L-1 NaF for 60 days. After the exposure treatment, the level of T-AOC, ROS and MDA, the activity of GPx, GST and GR and gene transcription of GPx1, GPx4, GST-mu5 and GR in the testis were detected. The mitochondria, endoplasmic reticulum and Golgi apparatus were observed by electron microscope. Results were as follows： Compared with the control group, the contents of T-AOC was decreased, the contents of ROS and MDA were raised, and the activity of GPx, GST and GR were reduced, and the relative mRNA transcription of GPx1, GPx4, GST-mu5 and GR were down-regulated. It was showed that the antioxidant capacity of testis were decreased and in an obvious oxidative stress state, which related to the decrease of the enzyme activity of glutathione antioxidant system and its related gene transcription.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2017年第5期954-962,共9页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金(31201965)
山西农业大学青年拔尖创新人才支持计划项目(TYIT201408)
