摘要
采用盆栽试验,连续种植3批菜心,研究生物有机肥和EM菌剂对连作9 a和6 a的菜园土壤微生物群落结构和功能多样性的影响。结果表明:与化肥处理相比,施用生物有机肥能够提高连作土壤中细菌、放线菌、丛枝菌根真菌和革兰氏阴性细菌(G^-)的相对含量及G^-/G^+(革兰氏阴性细菌/革兰氏阳性细菌)比值,并降低土壤真菌的相对含量;随着种植批次的增加,土壤中G^-、G^+含量降低,G^-/G^+比值上升,生物有机肥和EM菌剂配施化肥处理G^-含量普遍高于化肥处理;与化肥处理相比,施用生物有机肥土壤微生物AWCD值、Simpson指数、McIntosh指数均提高,土壤微生物活性、多样性和均一度提高。
Vegetable Choi Sum was pot tried for three rounds of cropping to observe the effects of bio-organic fertilizer and EM microbial agent on microbial community structure and functional diversity of the soil in the vegetable garden for 9 and 6 years of continuous cropping. The results showed that the bio-organic fertilizer increased the relative contents of bacteria, actinomycetes, arbuscular mycorrhizal fungi and gram-negative bacteria, and the gram-negative bacteria/gram-positive bacteria ratio of continuous cropping soil, but reduced the relative content of fungi, as compared to the chemical fertilizer. With the more croppings the bio-fertilizer reduced the relative contents of gram-negative bacteria, gram-positive bacteria, increased the gram-negative bacteria/gram-positive bacteria ratio. The bio-organic fertilizer and the EM agents ＋chemical fertilizer treatment generally had a higher relative content of gram-negative bacteria than the chemical fertilizer treatment. Comparing with chemical fertilizer, the bio-fertilizer treatment had a higher average well color development （AWCD）, Simpson index and Mclntosh index of soil microbes as well as soil microbial activity, diversity and uniformity than the chemical treatment.
出处
《热带农业科学》
2017年第4期57-62,共6页
Chinese Journal of Tropical Agriculture
基金
现代农业产业技术体系专项资金(No.CARS-25-C-04)
广东省省级科技计划项目(No.2014A020208113)
关键词
生物有机肥
EM菌剂
土壤微生物
连作障碍
bio-organic fertilizer
EM microbial agent
soil microbes
continuous cropping obstacle
