摘要
以金叶锦带的腋芽为外植体,采用组织培养法,通过在培养基中添加不同浓度激素,筛选最佳培养基,为金叶锦带的大面积推广提供技术支撑。结果表明:最佳诱导培养基为MS+0.6mg·L^(-1) 6-BA+0.1mg·L^(-1) NAA+蔗糖30g·L^(-1)+琼脂7g·L^(-1),诱导率达86.3%;最佳增殖培养基为MS+1.0 mg·L^(-1) 6-BA+1.0 mg·L^(-1) GA3+蔗糖30g·L^(-1)+琼脂7g·L^(-1),增殖系数7.250 6;最佳生根培养基为1/2MS+1.0mg·L^(-1) IBA+蔗糖30g·L^(-1)+琼脂7g·L^(-1),生根率100.0%。
Buds of Weigela ‘Red Prince’＋vat were used as explants.By using tissue culture methods，the optimum medium for Weigela ‘Red Prince’＋vat were selected via adding different concentrations of hormone,to provide technique reference for promotion of Weigela‘Red Prince’＋vat.The results showed that the inducing culture medium of MS＋0.6 mg·L^-1 6-BA＋0.1 mg·L^-1 NAA＋sucrose 30 g·L^-1＋agar 7 g·L^-1 was optimal for inducement，the inducement rate was 86.3%;the best proliferation medium was MS＋1.0 mg·L^-1 6-BA＋1.0 mg·L^-1 GA3＋sucrose 30 g·L^-1＋agar 7 g·L^-1，the proliferation times was 7.250 6;the best rooting medium formula was 1/2MS＋1.0 mg·L^-1 IBA＋sucrose 30 g·L^-1＋agar 7 g·L^-1,the rooting rate was 100.0%.
出处
《北方园艺》
CAS
北大核心
2017年第10期92-94,共3页
Northern Horticulture
基金
河北省科技厅科学研究计划资助项目(15236830)
关键词
金叶锦带
组织培养
激素配比
Weigela‘Red Prince’＋vat
tissue culture
hormone combination
