摘要
该文建立一种同时测定4种虫草类药材(冬虫夏草、蛹虫草、虫草菌丝体和蝉花)中虫草素(3'-脱氧腺苷)和2'-脱氧腺苷含量的在线固相萃取-高效液相色谱分析方法。供试品经ZORBAX SB-AQ(4.6 mm×12.5 mm,5μm)色谱柱富集,水-甲醇(91∶9)洗脱;ZORBAX SB-AQ(4.6 mm×150 mm,5μm)色谱柱分离,流动相为0.1%甲酸水溶液-甲醇(91∶9),检测波长260nm,柱温40℃,流速1.0 m L·min^(-1),分别对4种虫草类药材中虫草素和2'-脱氧腺苷含量进行检测。结果表明冬虫夏草、虫草菌丝体和蝉花样品中仅检测到2'-脱氧腺苷,蛹虫草样品中仅检测到虫草素,早期报道的在冬虫夏草中检测到的虫草素色谱峰可能是其同分异构体2'-脱氧腺苷色谱峰或以2'-脱氧腺苷为主的与虫草素的共洗脱色谱峰。该方法重复性好,准确度高,分析时间短,可用于虫草类药材中虫草素和2'-脱氧腺苷的分析。
An online SPE-HPLC method for simultaneous determination of cordycepin （3′-deoxyadenosine） and 2′-deoxyadenosine in Cordyceps genus （C. sinensis,C. militaris,Hirsutella sinensis and C. sobolifera） was developed. The samples were enriched on a ZORBAX SB-AQ （4.6 mm×12.5 mm,5 μm） column with isocratic elution by 9% methanol solution. The separation of analytes was performed on a ZORBAX SB-AQ （4.6 mm×150 mm,5 μm） column with gradient elution by 0.1% formic acid solution and methanol （91：9）. The flow rate was 1.0 mL·min^-1. Column temperature was 40℃ and detection wavelength was 260 nm. This method has been applied for analysis of different Cordyceps genus. The 2′-deoxyadenosine was detected in C. sinensis,Hirsutella sinensis and C. sobolifera. The cordycepin was detected in C. militaris. In summary,the cordycepin chromatographic peak from C. sinensis in some past reports may be the 2′-deoxyadenosine chromatographic peak or the mixture peak of 2′-deoxyadenosine and cordycepin in which 2′-deoxyadenosine content was higher than cordycepin. The developed method is suitable for analysis of cordycepin and 2′-deoxyadenosine in Cordyceps genus.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2017年第10期1932-1938,共7页
China Journal of Chinese Materia Medica
基金
国家工信部中药材生产扶持项目
