摘要
目的:研究杜仲中各成分对MC3T3-E1 Subclone 14成骨细胞增殖、成骨细胞保护素(DPG)与核因子κB受体活化因子配体(RANKL)比值的影响。方法:对杜仲皮进行组分提取,得到A组分(水洗脱液)、b组分(20%乙醇洗脱液)和C组分(40%乙醇洗脱液);对照组MC3T3-E1 Subclone 14成骨细胞加入DMEM/F-2培养基(Hyclon)培养,给药组分别加入浓度为200、100、50、25和12.5 mg/L含A、B、C组分的培养基培养;采用MTT法检测MC3T3-E1 Subclone 14成骨细胞增殖情况;ELISA法检测MC3T3-E1 Subclone 14成骨细胞OPG及RANKL活性,紫外分光光度法检测MC3T3-E1 Subclone 14成骨细胞碱性磷酸酶(ALP)活性。结果:与对照组比较,给药24 h后,100和200 mg/L C组分给药组的OD值明显增高(P<0.05);与对照组比较,给药48 h后,200 mg/LC组ALP的活性以及OPG与RANKL比值明显增高,差异具有统计学意义(P<0.05)。结论:杜仲40%乙醇提取组分能明显上调OPG与RANKL比值,并能促进MC3T3-E1 Subclone 14成骨细胞的增殖。
Objective： To screen effective components of Eucommia ulmoides for studying their el- rents on the proliferation of osteoblasts and ratio of OPG/RANKL in MC3T3-E1 Subelone 14 osteo- blasts. Methods： Extracting component of Eucommia, and dividing into A group （water washing liq- uid）, B group （20% ethanol washing liquid） and C group （ 40% ethanol washing liquid） ; control group MC3T3-E1 Subclone 14 osteoblasts was added with DMEM/F-2 culture medium（ Hyclon）, other groups were added with A, B, C components at the concentration of 200, 100, 50, 25 and 12.5 rag/L; MTr detection method to detect cell proliferation; the activity of OPG and RANKL are detected by ELISA ; UV spectrophotometry was adopted to detect the ALP activity of MC3T3-E1 Subclone 14 osteo- blasts. Results： Comparing with control group, 24 hr after medication, OD value of 100 and 200 mg/ L C group significantly increased （P 〈 0.05 ） ; comparing with control group, 48 hr after medication, ALP activity and OPG/RANKL ratio of 200 mg/L C group significantly increased, differences were sta- tistically signifieant（P 〈0.05 ）. Conclusion： 40% ethanol Eueommia ulmoides Oliv extract can upregulate the OPG/RANKL ratio, and enhance osteoblast proliferation.
出处
《贵州医科大学学报》
CAS
2017年第5期553-556,共4页
Journal of Guizhou Medical University
基金
贵州省科技重大专项[黔科合重大专项字(2012)6009号]
贵州省中药现代化专项项目[黔科合中药字(2013)5062号]
关键词
杜仲
成骨细胞
骨保护素
碱性磷酸酶
Eucommia ulmoides Oliv
osteoblasts
osteoprote-gerin
alkaline phosphatase
