摘要
采用超高效液相色谱法测定羊栖菜中岩藻甾醇的含量。羊栖菜试样(2.000 0g)经甲醇-二氯甲烷(1+1)混合液提取,所得提取液以YMC-Pack ODS-A色谱柱为分离柱,以甲醇-乙腈(3+7)混合液为流动相,在检测波长210nm处进行测定。岩藻甾醇的质量浓度在0.5~400mg·L^(-1)内与其对应的峰面积呈线性关系,检出限(3S/N)为0.16mg·L^(-1)。在25,50,100mg·L^(-1)等3个浓度水平进行加标回收试验,回收率在91.2%~96.8%之间。测定值的日内及日间相对标准偏差(n=6)分别在1.1%~5.4%,2.8%~7.0%之间。
UHPLC was applied to the determination of fucosterol in Sargassum fusiforme. The Sargassum fusiforme sample （2. 000 0 g） was extracted with mixed solution of methanol and dichloromethane （1＋ 1）. In the HPLC separation YMC-Pack ODS-A chromatographic column was used as stationary phase, and a mixture of methanol and acetonitrile （3＋7） was used as mobile phase. UV-detection at 210 nm was adopted in the determination. Linear relationship between values of peak area and mass concentration of fucosterol was kept in the range of 0. 5-400 mg·L^-1 with detection limit （3S/N） of 0. 16 mg·L^-1. Tests for recovery were made by standard addition method at the concentration levels of 25, 50, 100 mg·L^-1, giving values of recovery in the range of 91.2%-96.8%. Values of intra-day an inte-day RSD＇s （n=6） found were in the ranges of 1.1%-5.4%, and 2.80/00--7.0% respectively.
出处
《理化检验（化学分册）》
CSCD
北大核心
2017年第5期548-551,共4页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
基金
浙江自然科学基金项目(LY15C200004)
