摘要
目的观察白藜芦醇(RV)对慢性哮喘小鼠模型气道重塑的影响。方法 24只雌性BALB/c小鼠随机分成3组,即对照组、哮喘组、RV组,每组8只,3组小鼠均给予鸡卵清蛋白(OVA)致敏,对照组予以磷酸盐缓冲溶液(PBS)激发,哮喘组和RV组予以OVA激发,其中RV组于每次激发前30 min给予腹腔注射溶于二甲基亚砜(DMSO)的RV,对照组和哮喘组给予腹腔注射等量的DMSO。最后一次激发24 h后,取小鼠肺组织固定、切片行过碘酸希夫(PAS)染色、Masson染色及α-平滑肌肌动蛋白(α-SMA)免疫组织化学检测,分别检测杯状细胞增生、胶原沉积和平滑肌细胞增生肥大程度,结果用杯状细胞(PAS阳性)、胶原(Masson阳性)和平滑肌细胞(α-SMA阳性)阳染面积/支气管基底膜周长(μm2/μm)表示。结果对照组未见PAS+区域,哮喘组和RV组杯状细胞增生显著高于对照组(5.44±1.13、4.18±0.85比0.00±0.00,P均<0.01),且RV组显著低于哮喘组(P<0.05)。Masson染色提示哮喘组和RV组上皮下胶原沉积显著高于对照组(9.80±2.78、5.71±0.68比1.67±0.65,均P<0.01),且RV组显著低于哮喘组(P<0.01)。α-SMA免疫组化显示哮喘组和RV组上皮下胶原沉积显著高于对照组(10.39±1.65、7.57±1.98比2.41±1.06,均P<0.01),且RV组显著低于哮喘组(P<0.05)。结论 RV干预后,小鼠慢性哮喘模型气道重塑指标均有减轻,提示RV可能对慢性哮喘小鼠气道重塑具有抑制作用。
ObjectiveTo investigate the effects of resveratrol on airway remodeling in mice with chronic asthma. MethodsTwenty-four female BALB/c mice were randomly divided into three groups （8 mice in each group）, namely a control group, an asthma group and a resveratrol （RV） group. All mice were sensitized with ovalbumin （OVA）. The sensitized mice were then challenged with OVA while the control group were challenged with phosphate-buffered saline. The mice in the RV group were intraperitoneally injected with RV 30 min before OVA challenge, while the mice in the control and the asthma group were intraperitoneally injected with equal volume of dimethylsulfoxide. Periodic acid-Schiff （PAS） staining was performed to evaluate goblet cell hyperplasia, and Masson-trichrome staining was used to evaluate the deposition of collagen matrix. In addition, immunohistochemical analysis of the α-smooth muscle actin （α-SMA） was applied to examine airway smooth muscle cell hyperplasia and hypertrophy. The positive staining with PAS, Masson, α-SMA areas （μm 2/μm） of per bronchial basement membrane perimeter was used to indicate the degree of airway remodeling. ResultsIn the asthma group and the RV group, the degree of the goblet cell hyperplasia was significantly higher than that in the control group （5.44±1.13, 4.18±0.85vs. 0.00±0.00,P〈0.01）, and the level of goblet cell hyperplasia in the RV group was lower than that in the asthma group （P〈0.05）. The Masson staining showed that the deposition of collagen in the asthma group and the RV group was significantly higher than that in the control group （9.80±2.78, 5.71±0.68vs. 1.67±0.65,P〈0.01）, and the collagen deposition in the RV group was further lower than that in the asthma group （P〈0.01）. The α-SMA immunohistochemical analysis demonstrated that the expression of α-SMA in the asthma group and the RV group was significantly higher than that in the control group （10.39±1.65, 7.57±1.98vs. 2.41±1.06,P〈0.01）, and the level of α-SMA in the RV group was also lower than that in the asthma group （P〈0.05）. ConclusionThese findings suggest that resveratrol has an inhibitory effect on the process of airway remodeling in mice with chronic asthma.
出处
《中国呼吸与危重监护杂志》
CAS
CSCD
北大核心
2017年第3期223-227,共5页
Chinese Journal of Respiratory and Critical Care Medicine
基金
国家自然科学基金面上项目(81072684)
湖北省自然科学基金面上项目(2014CFB202)
