摘要
目的:研究尖锐湿疣病灶中hTERC、FOXP3基因扩增对免疫应答、细胞凋亡的影响。方法:收集在本院确诊的尖锐湿疣病灶以及同期接受包皮环切术的正常皮肤组织,抽提RNA后测定hTERC、FOXP3以及凋亡相关基因的mRNA扩增量,抽提蛋白后测定免疫应答相关细胞因子的蛋白含量。结果:尖锐湿疣病灶中hTERC、FOXP3、Livin、Survivin基因的mRNA扩增量以及IL-4、IL-10的蛋白含量均显著高于正常皮肤组织,TRAIL、Caspase-3、PDCD4的mRNA扩增量以及IL-2、TNF-α的蛋白含量均显著低于正常皮肤组织;FOXP3 mRNA高表达的尖锐湿疣病灶中IL-2、TNF-α的蛋白含量均显著低于FOXP3mRNA低表达的尖锐湿疣病灶,IL-4、IL-10的蛋白含量均显著高于FOXP3mRNA低表达的尖锐湿疣病灶;hTERC mRNA高表达的尖锐湿疣病灶中Livin、Survivin的mRNA扩增量均显著高于hTERC mRNA低表达的尖锐湿疣病灶,TRAIL、Caspase-3、PDCD4的mRNA扩增量均显著低于hTERC mRNA低表达的尖锐湿疣病灶。结论:尖锐湿疣病灶中hTERC、FOXP3基因的高表达分别具有抑制细胞凋亡和抑制抗病毒免疫应答的作用。
Objective： To study the effect of hTERC and FOXP3 gene amplification in condyloma acuminate lesions on the immune response and cell apoptosis. Methods： The condyloma acuminate lesions diagnosed between May 2014 and October 2016 and the normal skin tissue from circumcision during the same period were collected to extract RNA and then determine hTERC, FOXP3 and apoptosis-related gene mRNA amplification, and after protein extraction, the protein levels of immune response-related eytokines were determined. Results： hTERC, FOXP3, Livin and Survivin gene mRNA amplification as well as IL-4 and IL-10 protein levels in eondyloma acuminatum lesions were significantly higher than those in normal skin tissue while TRAIL, Caspase-3 and PDCD4 mRNA amplification as well as IL-2 and TNF-α protein levels were significantly lower than those in normal skin tissue; IL-2 and TNF-α protein levels in condyloma acuminatum lesions with high FOXP3 mRNA expression were significantly lower than those in condyloma acuminatum lesions with low FOXP3 mRNA expression while IL-4 and IL-10 protein levels were significantly higher than those in condyloma acuminatum lesions with low FOXP3 mRNA expression; Livin and Survivin mRNA amplification in condyloma acuminatum lesions with high hTERC mRNA expression were significantly higher than those in condyloma acuminatum lesions with low hTERC mRNA expression while TRAIL, Caspase-3 and PDCD4 mRNA amplification were significantly lower than those in condyloma acuminatum lesions with low hTERC mRNA ex pression. Conclusions： Highly expressed hTERC and FOXP3 genes in condyloma acuminatum lesions can inhibit apoptosis and inhibit antiviral immune response respectively.
出处
《海南医学院学报》
CAS
2017年第8期1142-1145,共4页
Journal of Hainan Medical University
基金
四川省卫生和计划生育委员会基金项目(30305030127)~~
